Strain Name:
B6.129S4-Ptpn11tm5Bgn/Mmjax
Stock Number:
032102-JAX
Citation ID:
RRID:MMRRC_032102-JAX

Gene Information

Ptpn11tm5Bgn
Name: protein tyrosine phosphatase, non-receptor type 11; targeted mutation 5, Benjamin Neel
Synonyms: Shp2 Ex2*, Ptpn11tm1Yan
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
Ptpn11
Name: protein tyrosine phosphatase, non-receptor type 11
Synonyms: PTP2C, PTP1D, SH-PTP2, SHP-2, Syp, Shp2, SH2 domain-containing protein tyrosine phosphatase-2, 2700084A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
NCBI: 19247
HGNC: HGNC:9644
Homologene: 2122
Genetic Alterations
A neo cassette was inserted into the place where exon2 was.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
Homozygous: Few embryos homozygous for the targeted mutation are observed after E6.5 or E7.5. Embryos that do implant are poorly organized, necrotic and display little appropriate tissue differentiation. Cultured blastocysts exhibit substantial cell loss after day 3 and exhibit a thin epithelioid layer instead of trophoplastic giant cells and fail to yield trophoblast stem cells. Blastocysts die as a result of apoptosis. This mutant mouse strain may be useful in studies of embryogenesis and stem cell survival.

Heterozygous: Mice are Mendelian and appear normal & fertile.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Mammalian Phenotype Terms
Allelic Composition: Ptpn11tm5Bgn/Ptpn11tm5Bgn (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

MeSH Terms
  • Animals
  • Apoptosis/physiology
  • Apoptosis Regulatory Proteins/genetics
  • Apoptosis Regulatory Proteins/metabolism
  • Bcl-2-Like Protein 11
  • Cell Survival
  • Cells, Cultured
  • Enzyme Activation
  • Extracellular Signal-Regulated MAP Kinases/genetics
  • Extracellular Signal-Regulated MAP Kinases/metabolism
  • Female
  • Fibroblast Growth Factor 4/metabolism
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Male
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatases/genetics
  • Protein Tyrosine Phosphatases/metabolism
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • SH2 Domain-Containing Protein Tyrosine Phosphatases
  • Signal Transduction/physiology
  • Stem Cells/cytology
  • Stem Cells/physiology
  • Trophoblasts/cytology
  • Trophoblasts/metabolism
  • ras Proteins/genetics
  • ras Proteins/metabolism
  • src Homology Domains
Strain Development
A targeting vector containing a splice acceptor, beta-gal, PGK neomycin cassette was used to replace exon 2 and the flanking intronic sequences. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 for 20 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Developmental Biology
Donor
Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute
Primary Reference
Yang W, Klaman LD, Chen B, Araki T, Harada H, Thomas SM, George EL, Neel BG. An Shp2/SFK/Ras/Erk signaling pathway controls trophoblast stem cell survival. Dev Cell. 2006 Mar;10(3):317-27. (Medline PMID: 16516835)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032102-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032102-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.