Strain Name:
B6.129S6-Ptpn11tm6Bgn/Mmjax
Stock Number:
032103-JAX
Citation ID:
RRID:MMRRC_032103-JAX

Strain Information

Ptpn11tm6Bgn
Name: protein tyrosine phosphatase, non-receptor type 11; targeted mutation 6, Benjamin Neel
Synonyms: Ptpn11inD61Y, inDY, Ptpn11tm1Toa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
Ptpn11
Name: protein tyrosine phosphatase, non-receptor type 11
Synonyms: 2700084A17Rik, Syp, SHP-2, SH-PTP2, PTP1D, PTP2C, Shp2, SH2 domain-containing protein tyrosine phosphatase-2
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Targeted Mutation
NCBI: 19247
HGNC: HGNC:9644
Homologene: 2122
Genetic Alterations
A point mutation of D61Y and a restriction enzyme site was inserted into exon3. By homologous recombination & subsequent removal of the neo cassette that was present & flanked by loxP site in exon 3.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Mice that are homozygous for the targeted mutation are not viable.

Heterozygous: hen used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study cardiac defects, fatal myeloproliferative disorder (MPD) and juvenile myelomonocytic leukemia (JMML).
MeSH Terms
  • Adoptive Transfer
  • Animals
  • Bone Marrow/metabolism
  • Bone Marrow/pathology
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Erythrocytes/metabolism
  • Erythrocytes/pathology
  • Erythroid Precursor Cells/metabolism
  • Erythroid Precursor Cells/pathology
  • Female
  • Flow Cytometry
  • Gene Knock-In Techniques
  • Genes, Lethal/physiology
  • Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
  • Granulocyte-Macrophage Progenitor Cells/metabolism
  • Granulocyte-Macrophage Progenitor Cells/pathology
  • Granulocytes/metabolism
  • Granulocytes/pathology
  • Hematopoiesis/physiology
  • Hematopoietic Stem Cells/metabolism
  • Hematopoietic Stem Cells/pathology
  • Integrases/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mitogen-Activated Protein Kinase 1/metabolism
  • Mitogen-Activated Protein Kinase 3/metabolism
  • Monocytes/metabolism
  • Monocytes/pathology
  • Myeloproliferative Disorders/etiology
  • Myeloproliferative Disorders/metabolism
  • Myeloproliferative Disorders/pathology
  • Phenotype
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11/physiology
  • STAT5 Transcription Factor/metabolism
  • Spleen/metabolism
  • Spleen/pathology
Strain Development
A targeting vector was designed to place a loxP-neo-loxP-STOP-loxP cassette between exons 2 and 3 and to insert a D61Y point mutation and unique AgeI restriction enzyme site in exon 3 of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for a minimum of 10 generations. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
This strain contains the same mutation as MMRRC:036173, 129S6-Ptpn11tm1Bgn/Mmucd.
Suggested Control Mice
Wild-type littermates or C57BL/6 mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
  • Hematology
  • Models for Human Disease
Donor
Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute
Primary Reference
Chan G, Kalaitzidis D, Usenko T, Kutok JL, Yang W, Mohi MG, Neel BG. Leukemogenic Ptpn11 causes fatal myeloproliferative disorder via cell-autonomous effects on multiple stages of hematopoiesis. Blood. 2009 Apr 30;113(18):4414-24. Epub 2009 Jan 29. (Medline PMID: 19179468)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
Generation
N11+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 12
Average litter size
7
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032103-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032103-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.