Strain Name:
B6.129S5-Skap2Gt(VICTR20)21Lex/Mmjax
Stock Number:
032106-JAX
Citation ID:
RRID:MMRRC_032106-JAX
Major Collection:

Gene Information

Skap2Gt(VICTR20)21Lex
Name: src family associated phosphoprotein 2; gene trap 21, Lexicon Genetics
Synonyms: SKAP-HOM-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
Skap2
Name: src family associated phosphoprotein 2
Synonyms: RA70, Saps, mSKAP55R, 2610021A10Rik, SKAP-HOM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
NCBI: 54353
Homologene: 2919
Genotype Determination
ES Cell Line
Lexicon cell line derived from 129S5/SvEvBrd
Phenotype
Homozygous: BCR-mediated proliferation is strongly attenuated in ko mice adhesion of activated B cell to fibronectin as well as to ICAM-1 is strongly reduced.

Heterozygous: normal
Mammalian Phenotype Terms
Allelic Composition: Skap2Gt(VICTR20)21Lex/Skap2Gt(VICTR20)21Lex (Genetic Background: B6.129S5-Skap2Gt(VICTR20)21Lex )

MeSH Terms
  • Adaptor Proteins, Signal Transducing/analysis
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/physiology
  • Animals
  • B-Lymphocytes/immunology
  • Cell Adhesion/immunology
  • Cytosol/chemistry
  • Cytosol/metabolism
  • Hematopoietic System/cytology
  • Hematopoietic System/metabolism
  • Immunoglobulins/blood
  • Integrins/metabolism
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Mice, Mutant Strains
  • Mitogen-Activated Protein Kinases/metabolism
  • Myelin Proteins
  • Myelin-Associated Glycoprotein/immunology
  • Myelin-Oligodendrocyte Glycoprotein
  • Phosphoproteins/analysis
  • Phosphoproteins/genetics
  • Phosphoproteins/physiology
  • Receptors, Antigen, B-Cell/metabolism
  • Signal Transduction
Strain Development
This deficient mouse was generated by retrovirus based gene trap technology.
Suggested Control Mice
Wild-type littermates
  • Immunology and Inflammation

Donor
Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute
Primary Reference
Togni M, Swanson KD, Reimann S, Kliche S, Pearce AC, Simeoni L, Reinhold D, Wienands J, Neel BG, Schraven B, Gerber A. Regulation of in vitro and in vivo immune functions by the cytosolic adaptor protein SKAP-HOM. Mol Cell Biol. 2005 Sep;25(18):8052-63. (Medline PMID: 16135797)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032106-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032106-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.