Strain Name:
STOCK Psap tm1Mhor/Mmmh
Stock Number:
032232-MU
Citation ID:
RRID:MMRRC_032232-MU

Strain Information

Psaptm1Mhor
Name: prosaposin; targeted mutation 1, Mia Horowitz
Synonyms: Prodelta8
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Targeted Mutation
Psap
Name: prosaposin
Synonyms: SGP-1
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Targeted Mutation
NCBI: 19156
HGNC: HGNC:9498
Homologene: 37680
Genetic Alterations
A neo cassette flanked by two loxP sites was inserted between intron 7 and intron 8, deleting exon 8 of the mouse prosaposin gene.(Construction of the prosaposin gene targeting vector: A 4.5-kb HindIII-EcoRI fragment, spanning introns 3 to 7 (including exons 4 to 7) of the prosaposin gene, and a 2-kb Ecl136II (SacI) fragment, spanning introns 8 to 10 (including exons 9 and 10) of prosaposin, were isolated from a {lambda}-FIX II mouse genomic clone (originating from a 129/SvEv genomic library; Stratagene. HindIII-EcoRI and Ecl136II fragments were subcloned into pET-28 plasmids (Novagen) and were inserted as the 5' and 3' arms, respectively, in the loxP/PGK-neo/PGK-tk vector, in which the phosphoglycerate kinase-neomycin (PGK-neo) cassette is flanked by two loxP sites . The two arms were cloned on either side of the loxP/PGK-neo cassette, with the neo gene in the same transcriptional orientation as prosaposin. The 2-kb Ecl136II fragment was inserted into a Bsu15I blunt-ended site, and the 4.5-kb HindIII-EcoRI blunt-ended fragment was inserted into a blunt-ended BamHI site, yielding the targeting vector pPro{Delta}8neoKSloxpPNT, missing the last 360 bp of intron 7, exon 8, and the first 165 bp of intron 8.)
Genotype Determination
ES Cell Line
W4 derived from 129S6/SvEvTac
Phenotype
Homozygous: The exon 8 prosaposin mutant mice are healthy and fertile with no obvious phenotype. No changes were detected in prosaposin secretion or in accumulation and metabolism of gangliosides, sulfatides, neutral glycosphingolipids, neutral phospholipids, other neutral lipids, and ceramide. These data strongly indicate that the prosaposin variant containing the exon 8- encoded three amino acids is dispensable for normal mouse development and fertility as well as for prosaposin secretion and its lysosomal function, at least in the presence of the prosaposin variant missing the exon 8- encoded three amino acids.

Heterozygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Psaptm1Mhor/Psaptm1Mhor (Genetic Background: either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * Black Swiss) )

MeSH Terms
  • Alternative Splicing/genetics
  • Alternative Splicing/physiology
  • Animals
  • Asparagine/genetics
  • Embryo, Mammalian/cytology
  • Exons/genetics
  • Fertility/genetics
  • Fertility/physiology
  • Glutamine/genetics
  • Lipid Metabolism
  • Lipids/analysis
  • Lysosomes/physiology
  • Male
  • Mice/genetics
  • Mice/growth & development
  • Mice, Mutant Strains
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Protein Isoforms/physiology
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Saposins/genetics
  • Saposins/physiology
  • Saposins/secretion
  • Sequence Deletion
  • Stem Cells/metabolism
  • Testis/metabolism
  • Tissue Distribution
Strain Development
Gene-targeted 129/SvEv embryonic stem (ES) cells were produced by electroporating XhoI-linearized pPro? 8neoKSloxpPNT into W4 (ES) cells, followed by selection with G418 and gancyclovir. Two hundred fifty double-resistant colonies were generated and examined by Southern blot analysis. Five properly targeted ES cell lines were identified, and three were used to produce chimeric males by morula aggregation. Chimeras were crossed with either 129/SvEv or Swiss Webster (SW) females to produce heterozygous Pro?8neo mice.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
Donor
Mia Horowitz Mia, Ph.D., Tel Aviv University
Primary Reference
Cohen T, Auerbach W, Ravid L, Bodennec J, Fein A, Futerman AH, Joyner AL, Horowitz M. The exon 8-containing prosaposin gene splice variant is dispensable for mouse development, lysosomal function, and secretion. Mol Cell Biol. 2005 Mar;25(6):2431-40. (Medline PMID: 15743835)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032232-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032232-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.