Strain Name:
Stock Number:
Citation ID:

Gene Information

Name: programmed cell death 1 ligand 2; targeted mutation 2, Arlene H Sharpe
Synonyms: PD-L2-
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Knockout
Name: programmed cell death 1 ligand 2
Synonyms: B7-DC, F730015O22Rik, PD-L2, Btdc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
NCBI: 58205
Homologene: 10973
ES Cell Line
J1 derived from 129S4/SvJae
Homozygous: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Following subcutaneous innoculation with Leishmania mexicana, mutant mice exhibit larger lesions and greater parasite burdens than susceptible 129S4/SvJae controls. Although TH1/TH2 response is similar to control, mutant mice have elevated levels of IgM and IgG2a. This mutant mouse strain may be useful in studies of immune response.

Heterozygous: Not evaluated
Mammalian Phenotype Terms
Allelic Composition: Pdcd1lg2tm2Shr/Pdcd1lg2tm2Shr (Genetic Background: C.B6-Pdcd1lg2tm2Shr )

MeSH Terms
  • Animals
  • B7-1 Antigen/biosynthesis
  • B7-1 Antigen/genetics
  • B7-1 Antigen/physiology
  • B7-H1 Antigen
  • CD4-Positive T-Lymphocytes/metabolism
  • CD8-Positive T-Lymphocytes/metabolism
  • Cells, Cultured
  • Cytokines/antagonists & inhibitors
  • Cytokines/metabolism
  • Diabetes Mellitus, Type 1/genetics
  • Hematopoietic Stem Cells/metabolism
  • Immune Tolerance/genetics
  • Immune Tolerance/immunology
  • Interferon-gamma
  • Membrane Glycoproteins/biosynthesis
  • Membrane Glycoproteins/deficiency
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred NOD
  • Mice, Knockout
  • Mice, SCID
  • Organ Specificity/immunology
  • Peptides/deficiency
  • Peptides/genetics
  • Peptides/physiology
  • Programmed Cell Death 1 Ligand 2 Protein
  • T-Lymphocytes/immunology
  • T-Lymphocytes/metabolism
Strain Development
A targeting vector containing hygromycin resistance and herpes simplex virus thymidine kinase genes was used to replace the IgV exon of the targeted gene. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to 129S4/SvJae mice and heterozygotes interbred. Upon arrival to MMRRC, mice were bred to 129S4/SvJaeJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Immunology and Inflammation
  • Models for Human Disease
  • Virology
Arlene H. Sharpe, M.D., Ph.D., Harvard Medical School.
Primary Reference
Keir ME, Liang SC, Guleria I, Latchman YE, Qipo A, Albacker LA, Koulmanda M, Freeman GJ, Sayegh MH, Sharpe AH. Tissue expression of PD-L1 mediates peripheral T cell tolerance. J Exp Med. 2006 Apr 17;203(4):883-95. Epub 2006 Apr 10. (Medline PMID: 16606670)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Not specified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032235-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
032235-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.