Allele Symbol: Ptpn1tm1Bbk
Name: targeted mutation 1, Barabra B Kahn
Alteration at locus: Knockout
Gene Symbol: Ptpn1
Name: protein tyrosine phosphatase, non-receptor type 1
Alteration at locus: Knockout
ES Cell Line: J1 derived from 129S4/SvJae
Mice that are homozygous for the targeted mutation are viable and fertile. Homozygous males exhibit decreased body weight on a standard chow diet; weight gain in females is comparable to controls. On a 55% fat diet, both males and females remain lean. Although adipocyte cell number is not decreased, adipocyte volume is significantly decreased. Basal metabolic rate and total energy expenditure are increased in male homozygotes on high fat diet. Additionally, male homozygous mice exhibit decreased leptin levels, increased insulin sensitivity as a result of increased glucose utilization in skeletal muscle and enhanced glucose tolerance. This mutant mouse strain may be useful in studies of energy regulation, diet-induced obesity and glucose metabolism.
Heterozygous males exhibit decreased body weight on a standard chow diet; however, on a 55% fat diet weight gain is similar to wild-type. Leptin levels are decreased in heterozygous males on both chow and high-fat diets.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain of Origin: 129S4/SvJae
Strain genetic background: B6.129S4
Strain Development: A targeting vector containing a neomycin resistance cassette was used to replace the ATG-coding region in exon 1 and 2.3 kb of flanking sequence. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J for 20 generations. Upon arrival to MMRRC, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice: Wild-type littermates or C57BL/6J
Donor: Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email email@example.com.
Coat color: Black
MMRRC Breeding System: Backcross or Sib-mating
Overall Breeding Performance: Excellent
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||8 months||12 months|
Average litter size: 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.