Strain Name    : B6.129S4-Ptpn1tm1Bbk/Mmjax

Stock Number : 032240-JAX

Gene Information

(provided by MGI)
Allele Symbol: Ptpn1tm1Bbk
Name: targeted mutation 1, Barabra B Kahn
Chromosome: 2
Alteration at locus: Knockout
Gene Symbol: Ptpn1
Name: protein tyrosine phosphatase, non-receptor type 1
Chromosome: 2
Alteration at locus: Knockout
Genetic Alterations:
A neomycin cassette was inserted into exon 1.

Genotype Determination:

ES Cell Line: J1 derived from 129S4/SvJae

Phenotype

Mice that are homozygous for the targeted mutation are viable and fertile. Homozygous males exhibit decreased body weight on a standard chow diet; weight gain in females is comparable to controls. On a 55% fat diet, both males and females remain lean. Although adipocyte cell number is not decreased, adipocyte volume is significantly decreased. Basal metabolic rate and total energy expenditure are increased in male homozygotes on high fat diet. Additionally, male homozygous mice exhibit decreased leptin levels, increased insulin sensitivity as a result of increased glucose utilization in skeletal muscle and enhanced glucose tolerance. This mutant mouse strain may be useful in studies of energy regulation, diet-induced obesity and glucose metabolism.

Heterozygous males exhibit decreased body weight on a standard chow diet; however, on a 55% fat diet weight gain is similar to wild-type. Leptin levels are decreased in heterozygous males on both chow and high-fat diets.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Mammalian Phenotype Terms:
Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129/Sv * C57BL/6 )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1tm1Bbk (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

Allelic Composition: Ptpn1tm1Bbk/Ptpn1+ (Genetic Background: involves: 129S4/SvJae * C57BL/6J )

Strain of Origin: 129S4/SvJae

Strain genetic background: B6.129S4

Strain Development: A targeting vector containing a neomycin resistance cassette was used to replace the ATG-coding region in exon 1 and 2.3 kb of flanking sequence. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J for 20 generations. Upon arrival to MMRRC, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates or C57BL/6J

  • Diabetes/Obesity
  • Metabolism

Donor: Benjamin G. Neel, M.D., Ph.D., Ontario Cancer Institute.

Primary Reference:

  • Klaman LD, Boss O, Peroni OD, Kim JK, Martino JL, Zabolotny JM, Moghal N, Lubkin M, Kim YB, Sharpe AH, Stricker-Krongrad A, Shulman GI, Neel BG, Kahn BB. Increased energy expenditure, decreased adiposity, and tissue-specific insulin sensitivity in protein-tyrosine phosphatase 1B-deficient mice. Mol Cell Biol. 2000 Aug;20(15):5479-89. (Medline PMID: 10891488 )
  • Elchebly M; Payette P; Michaliszyn E; Cromlish W; Collins S; Loy AL; Normandin D; Cheng A; Himms-Hagen J; Chan CC; Ramachandran C; Gresser MJ; Tremblay ML; Kennedy BP, Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene [see comments], Science 1999 Mar 5;283(5407):1544-8 (Medline PMID: 10066179 )

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Coat color: Black

Other:

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
  • Homozygous female x Homozygous male littermates
  • While maintaining a live colony, these mice are bred as homozygotes.

Generation: N20+

Overall Breeding Performance: Excellent

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 12 months

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
032240-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032240-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.