Strain Detail Sheet

Strain Name    :

B6.129-B2mtm1Jae Tap1tm1Hpl/Mmucd

Stock Number :

032245-UCD

Other Names   :

B2m-/- Tap1-/-, B6.129-B2mtm1Jae Tap1tm1Hpl/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: B2mtm1Jae
Name: targeted mutation 1, Rudolf Jaenisch
Alteration at locus: Knockout
Allele Symbol: Tap1tm1Hpl
Name: targeted mutation 1, Hidde Ploegh
Alteration at locus: Knockout
Gene Symbol: B2m
Name: beta-2 microglobulin
Chromosome: 2
Alteration at locus: Knockout
Gene Symbol: Tap1
Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Chromosome: 17
Alteration at locus: Knockout

Genetic Alterations:
A neomycin resistance cassette was inserted into the second exon of B2m. Sequences encoding the second and part of the third transmembrane helices of Tap1 were replaced by a neomycin resistance gene.

Genotype Determination:

ES Cell Line: E14

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Low levels of MHC Class I on cell surface; impaired immune response; changes in hippocampal synaptic plasticity, homeostatic scaling, motor learning, pheromone- driven behavior, and motoneuron axon regeneration.

Heterozygous phenotype: Heterozygote seems to express normal levels of MHCI and appears like wild type mouse.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Tap1tm1Hpl/Tap1tm1Hpl
        B6.129-B2m<tm1Jae> Tap1<tm1Hpl>
  • nervous system phenotype
    • abnormal lateral geniculate nucleus morphology (MGI Ref ID J:66246)
      • area of the ipsilateral projection is increased compared to controls
      • in some cases, multiple ectopic clusters of inputs are present in the medial areas of the LGN
      • retinogeniculate axons, glomeruli and bouton appear normal
    • abnormal long term depression (MGI Ref ID J:66246)
      • 900 pulses at 1 Hz enhances baseline LTD, but LTD is unchanged in wild-type
      • reduced long term depression (MGI Ref ID J:66246)
        • 900 pulses at 0.5 Hz does not induce significant LTD in contrast to wild-type response
    • abnormal miniature excitatory postsynaptic currents (MGI Ref ID J:120941)
      • 40% increase in frequency in hippocampal neurons
      • 100% increase in frequency in layer 4 cortical neurons
      • median amplitude is similar to wild-type in hippocampal and layer 4 neurons
      • treatment with the neural activity blocker, Tetrodotoxin (TTX), does not increase mEPSC in mutant hippocampal cultures in contrast to increases in both amplitude and frequency observed in wild-type
    • abnormal synapse morphology (MGI Ref ID J:120941)
      • mice exhibit significantly fewer perforations at hippocampal synapses that wild-type
      • abnormal synaptic vesicle number (MGI Ref ID J:120941)
        • presynaptic nerve terminals contain 10% more synaptic vesicles than wild-type
    • abnormal synaptic bouton morphology (MGI Ref ID J:120941)
      • increase in size of excitatory presynaptic boutons
    • enhanced long term potentiation (MGI Ref ID J:66246)
      • LTP in response to tetanus is enhanced by approximately 227% as compared to wild type
    • enlarged lateral ventricles (MGI Ref ID J:66246)
      • 52% of P13 mice and 57% of adult mice have enlarged lateral ventricles

Strain of Origin: 129S2/SvPas x C57BL/6 (B2m) and 129P2/OlaHsd x C57BL/6 (Tap1)

Strain genetic background: C57BL/6

Suggested Control Mice:

  • Wild-type littermates

Research Applications

  • Immunology and Inflammation
  • Neurobiology

Strain Origin

Donor: Carla Shatz, Ph.D., Stanford University

Primary Reference:

  • Huh GS; Boulanger LM; Du H; Riquelme PA; Brotz TM; Shatz CJ, Functional requirement for class I MHC in CNS development and plasticity, Science 2000 Dec 15;290(5499):2155-9 (Medline PMID: 11118151)
  • Goddard CA; Butts DA; Shatz CJ, Regulation of CNS synapses by neuronal MHC class I., Proc Natl Acad Sci U S A 2007 Apr 17;104(16):6828-33 (Medline PMID: 17420446)

Colony and Husbandry Information

Special Considerations

No sib-mating; non-litter mates (cousins) of same generation are bred together.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
032245-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032245-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
032245-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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