Strain Name:
B6.129P2-PrepGt(RRM213)Byg/Mmucd
Stock Number:
032638-UCD
Citation ID:
RRID:MMRRC_032638-UCD
Other Names:
PREP-GT, B6.129P2-PrepGt(RRM213)Byg/Mmcd
Major Collection:

Strain Information

PrepGt(RRM213)Byg
Name: prolyl endopeptidase; gene trap RRM213, BayGenomics
Synonyms: PREPgt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Gene Trap
Prep
Name: prolyl endopeptidase
Synonyms: Pop, prolyl oligopeptidase, D10Wsu136e
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Gene Trap
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Genetic Alterations
Bay Genomics clone RRM213 has an insertion in the 2nd intron of the PREP gene in a 129- strain ES cell.
Genotype Determination
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype
Homozygous: Female body weight was lower than controls. BMI in males fed a high-fat diet was significantly smaller than WT/gt or WT/WT males

Heterozygous: WT/gt dams crossed with WT/WT sires reveal highly significant parent-of-origin effects in their progeny compared to the reciprocal cross (WT/WT dam x WT/gt sire). Body weight, adiposity index, body mass index, and liver, kidney and spleen weights are all significantly higher in pups born to WT/gt dams regardless of pup genotype.
Mammalian Phenotype Terms
Allelic Composition: PrepGt(RRM213)Byg/PrepGt(RRM213)Byg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: PrepGt(RRM213)Byg/Prep+ (Genetic Background: B6.129P2-PrepGt(RRM213)Byg )

Allelic Composition: PrepGt(RRM213)Byg/Prep+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

MeSH Terms
  • Animals
  • Cell Line
  • Databases, Nucleic Acid
  • Embryo, Mammalian/cytology
  • Genomics
  • Internet
  • Mice/genetics
  • Mice, Knockout
  • Mutagenesis, Insertional
  • Mutation
  • Stem Cells/cytology
  • User-Computer Interface
Strain Development
Received 12 chimeric founders. Selected 6 with the highest percentage of 129 chimerism visibly present (lightest patchwork colors) as an indicator of greatest incorporation and presence of 129-strain ES cells containing the RRM213 clone. Backcrossed to B6 until N8. Intercrossed N8 mice to generate homozygous gt/gt progeny. gt/gt mice bred to each other to maintain homozygous PREP-GT colony via random intra-strain mating.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
  • Neurobiology
Donor
Craig Warden, Ph.D., University of California, Davis
Primary Reference
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res. 2003 Jan 1;31(1):278-81. (Medline PMID: 12520002)

Colony and Husbandry Information

In donor's experience, PREP-GT dams tend to be more nervous. They like privacy and always build very elaborate nests that completely or almost completely hide their litters from view/light. Donor gave them cotton nestlets and mouse igloos as enrichment. Nests will be built up with cotton nestlet and bedding at the back corner of the cage, or the mouse igloo will be used at the front of the cage but only after packing the igloo with bedding which obstructs view/light. PREP-GT dams also seem to be more sensitive to working noise levels than any of our other B6-derived strains. Too much noise or repeated disturbance of their privacy appears to result in higher pup mortality.

In donor's animal rooms, light cycle is 14h light/10h dark, ambient temperature is 21C +/- 2C, and > 25% humidity. All breeding pairs and their pre-wean litters were housed in shoebox cages w/ DI H2O bottles, receive autoclaved cotton nestlets and mouse igloos as enrichment, and are fed LabDiet chow 5015. All non-breeding mice were housed in shoebox cages w/ DI H2O bottles or automatic watering, receive autoclaved torn pieces of egg-tray flats as enrichment, and were fed purified experimental diet or LabDiet chow 5001. Any detrimental effects on mice NOT given the above treatments are unknown.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032638-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032638-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
032638-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.