Strain Name:
B6.Cg-Lipctm1Unc Tg(Lipc)1Chw/Mmucd
Stock Number:
032639-UCD
Citation ID:
RRID:MMRRC_032639-UCD
Other Names:
SPRET-HL transgenic, B6.Cg-Lipctm1Unc Tg(Lipc)1Chw/Mmcd

Strain Information

Lipctm1Unc
Name: lipase, hepatic; targeted mutation 1, University of North Carolina
Synonyms: HL-deficient, hl-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Lipc
Name: lipase, hepatic
Synonyms: HL, Hpl
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 15450
VEGA: 9
HGNC: HGNC:6619
Homologene: 199
Tg(Lipc)1Chw
Name: transgene insertion 1, Craig Warden
Synonyms: SPRET-HL transgenic
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
BAC clone containing SPRET hepatic lipase sequence was microinjected into B6CBA F1/J cells. All data suggest a single integration site of the full length clone into B6 chromosome.
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Phenotype
Homozygous: Undetermined.

Heterozygous: Significant allele effects were observed such that compared to wildtype, SPRET-HL transgenics mediated significant reductions in HDL-triglyceride (TG) and VLDL-, LDL-, HDL-, and total cholesterol (TC) in fed males. SPRET-HL also significantly decreased VLDL-, HDL-, and total TG levels in fasted males. Fasted female transgenics had reduced TC compared to wildtype. Donor also found allele and sex effects on lipoprotein particle size. Male transgenic mice had increased VLDL and decreased LDL size, and female transgenic mice had decreased HDL size compared to control animals. Importantly, differences in HL activity levels did not mediate the changes in plasma lipids and lipoproteins. Moreover, donor observed dramatic differences in phospholipids and in PC and PE concentrations that were dependent on which allele (SPRET vs. B6) was expressed.
Mammalian Phenotype Terms
Allelic Composition: Lipctm1Unc/Lipctm1Unc (Genetic Background: B6.129P2-Lipctm1Unc )

Allelic Composition: Lipctm1Unc/Lipctm1Unc (Genetic Background: involves: 129 )

Allelic Composition: Lipctm1Unc/Lipctm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

MeSH Terms
  • Alleles
  • Animals
  • Chromatography, High Pressure Liquid
  • Chromosomes, Artificial, Bacterial
  • Chromosomes, Mammalian/genetics
  • Female
  • Homozygote
  • Lipase/chemistry
  • Lipase/genetics
  • Lipase/metabolism
  • Lipoproteins/blood
  • Lipoproteins/chemistry
  • Lipoproteins/metabolism
  • Male
  • Mice
  • Mice, Transgenic
  • Obesity/genetics
  • Obesity/mortality
  • Obesity/physiopathology
  • Particle Size
  • Phenotype
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Structure-Activity Relationship
Strain Development
Received founders from Murine Targeted Genomics Laboratory at UC Davis. Three founders were positive for the full length BAC clone and were backcrossed for 2 generations to B6.129- Lipc/J (HL -/-). Only progeny from a single founder demonstrated HL activity on the HL-/- background. Descendants from this single founder have been backcrossed to HL -/- for 15 generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
Donor
Craig Warden, Ph.D., University of California, Davis
Primary Reference
Pratt SM, Chiu S, Espinal GM, Shibata NM, Wong H, Warden CH. Mouse hepatic lipase alleles with variable effects on lipoprotein composition and size. J Lipid Res. 2009 Nov 5. (Medline PMID: 19965617)

Colony and Husbandry Information

Donor normally used LabDiet 5015 for breeding mice to support the production of PPE litters. Because B6.129-Lipctm1Unc/J (HL-/-) females tend to become obese on this diet after nursing anywhere from 1 to 3 litters and subsequently stop breeding successfully, donor usually backcross Tg+ females to HL -/- males. Male HL -/- sires can also become obese on the 5015 diet over time, but this was not usually a problem because once there are enough progeny to support a small colony, donor moved on to breed the next generation.

In donor's animal rooms, light cycle is 14h light/10h dark, ambient temperature is 21C +/- 2C, and > 25% humidity. All breeding pairs and their pre-wean litters were housed in shoebox cages w/ DI H2O bottles, receive autoclaved cotton nestlets and mouse igloos as enrichment, and are fed LabDiet chow 5015. All non-breeding mice were housed in shoebox cages w/ DI H2O bottles or automatic watering, receive autoclaved torn pieces of egg-tray flats as enrichment, and were fed purified experimental diet or LabDiet chow 5001. Any detrimental effects on mice NOT given the above treatments are unknown.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032639-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032639-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
032639-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.