Strain Name    : B6.129X1-Runx2tm1Ldq/Mmmh

Stock Number : 032650-MU

Gene Information

(provided by MGI)
Allele Symbol: Runx2tm1Ldq
Name: targeted mutation 1, L Darryl Quarles
Chromosome: 17
Alteration at locus: Knockout
Gene Symbol: Runx2
Name: runt related transcription factor 2
Chromosome: 17
Alteration at locus: Knockout
Genetic Alterations:
Targeted deletion of Runx2-II isoform in Runx2 gene locus. The P1 promoter and Runx2-II exon 1 has been deleted in this strain.

Genotype Determination:

ES Cell Line: Not specified

Phenotype:

Homozygous phenotype: The homozygous Runx2-II knock-out mice are perinatal lethal, and have severe skeletal defects in both intramembraous and endochondral bone formation.

Heterozygous phenotype: The heterzygous phenotype in Runx2-II mice leads to low turn-over osteopenia in postnatal bone formation.

Mammalian Phenotype Terms:
Allelic Composition: Runx2tm1Ldq/Runx2tm1Ldq (Genetic Background: involves: C57BL/6J )

Allelic Composition: Runx2tm1Ldq/Runx2+ (Genetic Background: involves: C57BL/6J )

Strain of Origin: 129X1, B6 mix

Strain genetic background: C57BL/6J

Strain Development: The original founder strain has been backcrossed with C57BL/6J for 12 generations, and then the current strain has been maintained for 6 sib-mated generations.

Suggested Control Mice:

  • Wildtype littermates

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Research Tools

Donor: L. Darryl Quarles, M.D., University of Tennessee Health Science Center

Primary Reference:

  • Xiao ZS, Hjelmeland AB, Quarles LD. 2004, Selective deficiency of the "bone-related" Runx2-II unexpectedly preserves osteoblast-mediated skeletogenesis. J Biol Chem 279(19):20307- 20313. (Medline PMID: 15007057)
  • Xiao ZS, Awad, H.A., Liu, S., Mahlios, J., Zhang, S., Guilak, F., Mayo, M.S., and Quarles, L.D. 2005, Selective Runx2-II deficiency leads to low-turnover osteopenia in adult mice. Dev Biol 283:345-356. (Medline PMID: 15936013 )
  • Zhang SQ, Xiao ZS, Luo JM, He N, Mahlios J, Quarles L.D. 2009, Dose-dependent effects of Runx2 on bone development. J Bone Miner Res. Nov;24(11):1889-904. (Medline PMID: 19419310)

Colony and Husbandry Information

Sib-mating. Mice mated at ages between 6-24 weeks.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
032650-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032650-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.