Strain Detail Sheet

Strain Name    :

STOCK Grhl3tm1(cre)Cgh/Mmucd

Stock Number :

032799-UCD

Other Names   :

Grhl3-Cre, STOCK Grhl3tm1(cre)Cgh/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Grhl3tm1(cre)Cgh
Name: targeted mutation 1, Shaun R Coughlin
Alteration at locus: Knock-In
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Knock-In
Target: Grhl3
Name: grainyhead-like 3 (Drosophila)
Chromosome: 4
Alteration at locus: Knock-In

Genetic Alterations:
Cre recombinase introduced in frame to the start codon of Grhl3

Genotype Determination:

ES Cell Line: E14

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: embryonic lethal, spina bifuda, NTDs (neural tube defects)

Heterozygous phenotype: none


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Grhl3tm1(cre)Cgh/Grhl3tm1(cre)Cgh
        involves: 129P2/OlaHsd * C57BL/6 * SJL
  • embryogenesis phenotype
    • abnormal neural tube morphology/development (MGI Ref ID J:157446)
      • neural tube phenotypes are stated to be identical to that of Grhl3tm1Jane homozygotes; however no data are presented
      • spina bifida (MGI Ref ID J:157446)
  • nervous system phenotype
    • abnormal brain development (MGI Ref ID J:157446)
    • abnormal neural tube morphology/development (MGI Ref ID J:157446)
      • neural tube phenotypes are stated to be identical to that of Grhl3tm1Jane homozygotes; however no data are presented
      • spina bifida (MGI Ref ID J:157446)

Strain of Origin: 50% 129P2/OlaHsd: 50% C57Bl/6

Strain genetic background: FVB/N

Strain Development: Founder mice 50% 129P2/OlaHsd: 50% C57BL/6.Subsequent generations backcrossed to FVB/N for 3 generations. Sib matings to maintain line.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Research Tools

Strain Origin

Donor: Shaun Coughlin, M.D., Ph.D., University of California San Francisco

Primary Reference:

Camerer E; Barker A; Duong DN; Ganesan R; Kataoka H; Cornelissen I; Darragh MR; Hussain A; Zheng YW; Srinivasan Y; Brown C; Xu SM; Regard JB; Lin CY; Craik CS; Kirchhofer D; Coughlin SR, Local protease signaling contributes to neural tube closure in the mouse embryo., Dev Cell 2010 Jan 19;18(1):25-38 (Medline PMID: 20152175)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
032799-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032799-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
032799-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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