Strain Name:
STOCK Tg(Amelx-Amtn)57Mlp/Mmucd
Stock Number:
032818-UCD
Citation ID:
RRID:MMRRC_032818-UCD
Other Names:
TgAMTN 57 (high expression)

Strain Information

Amelx
Name: amelogenin, X-linked
Synonyms: Amg, Amel, M100888, Rgsc888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11704
Homologene: 47996
Amtn
Name: amelotin
Synonyms: 5430427O21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71421
Homologene: 52640
Tg(Amelx-Amtn)57Mlp
Name: transgene insertion 57, Michael L Paine
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The mouse amelogenin promoter was used in the construction of a plasmid cassette to express the amelotin protein.
Genotype Determination
Phenotype
Homozygous: These transgenic animals exhibit imperfections of their dental enamel that is evident at the macroscopic and microscopic level. The typical enamel "rod" morphology is not apparent in these animals.
MeSH Terms
  • Ameloblasts/metabolism
  • Ameloblasts/pathology
  • Amelogenin/genetics
  • Amelogenin/metabolism
  • Amino Acid Sequence
  • Animals
  • Dental Enamel/pathology
  • Dental Enamel/ultrastructure
  • Dental Enamel Proteins/genetics
  • Dental Enamel Proteins/metabolism
  • Durapatite/chemistry
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Promoter Regions, Genetic
Strain Development
Fertilized eggs for microinjection were harvested from superovulated six week old female B6D2F1/J mice impregnated by inbred adult male (CBA/J) mice. For embryo transfer, pseudo-pregnant females were produced by mating CD1 adult females with a vasectomized CD1 adult male. Once transgenic animals were identified (F0), the breeding was between siblings, and after F1, if posseble only hemizygous siblings were used to breed.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
    Donor
    Michael L. Paine, Ph.D., University of Southern California.
    Primary Reference
    Lacruz RS, Nakayama Y, Holcroft J, Nguyen V, Somogyi-Ganss E, Snead ML, White SN, Paine ML, Ganss B. Targeted overexpression of amelotin disrupts the microstructure of dental enamel. PLoS One. 2012;7(4):e35200. Epub 2012 Apr 23. (Medline PMID: 22539960).

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
    MMRRC Breeding System
    Sib-mating
    Breeding Scheme(s)
    • Hemizygous female x Hemizygous male
    Generation
    F7
    Overall Breeding Performance
    Excellent
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: 8 months 8 months
    Average litter size
    10
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    032818-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
    Non-Profit / For-Profit
    Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    032818-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    032818-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.