Strain Detail Sheet

Strain Name    :

B6.FVB-Tg(Per1-Venus)33Obr

Stock Number :

032820-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tg(Per1-Venus)33Obr
Name: transgene insertion 33, Karl Obrietan
Alteration at locus: Transgenic

Genetic Alterations:
A bacterial articificial chromosone (BAC) #RP24-277K16 containing the mouse Per1 gene was modified by the introduction of a Venus (yellow fluorescent protein)-NLS-PEST fusion gene. To improve the kinetics of YFP, the PEST domain of the mouse ornithine carboxylase gene was added to the penultimate codon of Venus and a nuclear localization sequence (NLS) was cloned in-frame between the Venus coding sequence and the PEST domain. The fusion gene contains an hCMV promoter and a SV40 polyadenylation site.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Not evaluated.

Hemizygous phenotype: Mice that are hemizygous for the clock fluorescent reporter transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The mPeriod1-Venus transgene has widespread expression in multiple brain regions (high expression in: suprachiasmatic nuclei (SCN), Purkinje cells, cerebellum, cerebral cortex and hippocampus) and parallels Per1 expression. In addition, the transgene recapitulates Per1 rhythmic and light-inducible expression in the SCN. This mutant mouse strain may be useful for in vivo imaging to monitor circadian clock timing.

Strain of Origin: FVB/N

Strain genetic background: B6.FVB

Strain Development: The BAC was microinjected into the pronuclei of oocytes from FVB/N females. Founder line 33 was established and bred to C57BL/6J mice to generate the colony. Mice were subsequently backcrossed to C57BL/6J mice for three generations. mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Karl Obrietan, Ph.D., Ohio State University

Primary Reference:

  • Cheng HY; Alvarez-Saavedra M; Dziema H; Choi YS; Li A; Obrietan K, Segregation of expression of mPeriod gene homologs in neurons and glia: possible divergent roles of mPeriod1 and mPeriod2 in the brain., Hum Mol Genet 2009 Aug 15;18(16):3110-24 (Medline PMID: 19477955)
  • Cheng HY, Papp JW, Varlamova O, Dziema H, Russell B, Curfman JP, Nakazawa T, Shimizu K, Okamura H, Impey S, Obrietan K. microRNA modulation of circadian-clock period and entrainment. Neuron. 2007 Jun 7;54(5):813-29. (Medline PMID: 17553428)

Colony and Husbandry Information

Special Considerations

While maintaining a live colony, these mice are bred as hemizygotes.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
032820-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032820-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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