Strain Name:
B6.129X1-Prkacatm3Gsm/Mmmh
Stock Number:
032825-MU
Citation ID:
RRID:MMRRC_032825-MU

Strain Information

Prkacatm3Gsm
Name: protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 3, G Stanley McKnight
Synonyms: CalphaR
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knock-In
Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knock-In
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574
Genetic Alterations
A tryptophan 196 to arginine mutation was introduced into exon 7 of the mouse Prkaca genome. This mutation creates a constitutively active catalytic subunit that is not inhibited by regulatory subunits. One additional point mutation at the wobble position of Thr 195 results in the creation of an Mlu I restriction site specific to the mutated allele. A loxP- flanked neomycin cassette containing the SV40 promoter, the neomycin phosphotransferase gene, and the SV40 polyadenylation sequences was inserted into a unique SnaBI restriction site within the intron between exons 5 and 6 and this prevents expression of the constitutive CaR allele. Cre recombinase activates the expression of the CaR allele.
Genotype Determination
ES Cell Line
REK3 derived from 129X1/SvJ
Phenotype
Homozygous: In the absence of Cre recombination the mutated Prkaca allele is inactive and homozygotes are null for Prkaca. The animals are runted with a high incidence of perinatal lethality.

Heterozygous: Mice heterozygous for the CaR allele of Prkaca are viable, healthy, and fertile.
Mammalian Phenotype Terms
Allelic Composition: Prkacatm3Gsm/Prkacatm3Gsm (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Animals
  • Base Sequence
  • Blood Glucose/metabolism
  • Cyclic AMP-Dependent Protein Kinase Type II
  • Cyclic AMP-Dependent Protein Kinases/genetics
  • Cyclic AMP-Dependent Protein Kinases/metabolism
  • Gene Expression Regulation
  • Gene Targeting
  • Glucose Metabolism Disorders/genetics
  • Glucose Metabolism Disorders/metabolism
  • Glucose Tolerance Test
  • Insulin/blood
  • Insulin/secretion
  • Integrases/genetics
  • Integrases/metabolism
  • Mice
  • Mutagenesis, Insertional
  • Mutation
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Cell Lineage/genetics
  • Chromosomes, Artificial, Bacterial
  • Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/genetics
  • Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/metabolism
  • Gene Expression Regulation, Developmental
  • Genotype
  • Hair Color/genetics
  • Melanins/metabolism
  • Melanocytes/enzymology
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Microphthalmia-Associated Transcription Factor/genetics
  • Phenotype
  • Proteins/genetics
  • Proteins/metabolism
  • RNA, Untranslated
  • Recombination, Genetic
  • Regulatory Elements, Transcriptional
  • Signal Transduction/genetics
Strain Development
Once F1 animals were obtained by breeding chimeras to C57BL/6Crl (Charles River Labs), animals have been continously backcrossed to C57BL/6Crl for at least twenty generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
  • Sensorineural
  • Virology
Donor
G. Stanley McKnight, Ph.D. University of Washington
Primary Reference
  • Niswender CM; Willis BS; Wallen A; Sweet IR; Jetton TL; Thompson BR; Wu C; Lange AJ; McKnight GS, Cre recombinase-dependent expression of a constitutively active mutant allele of the catalytic subunit of protein kinase A., Genesis 2005 Nov;43(3):109-19. (Medline PMID: 16155866)
  • Alizadeh A; Fitch KR; Niswender CM; McKnight GS; Barsh GS, Melanocyte-lineage expression of Cre recombinase using Mitf regulatory elements., Pigment Cell Melanoma Res 2008 Feb;21(1):63-9. (Medline PMID: 18353144)

Colony and Husbandry Information

Animals are functionally heterozygous at the Prkaca locus and show no overt phenotypes and can be treated like any normal health mouse strain. Homozygous mice cannot be made, as loss of Prkaca will lead to animals that are born but have growth defects and fail to thrive.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032825-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032825-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.