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Strain Detail Sheet

Strain Name:
B6.129P2(Cg)-Hprt1tm8(Ple151-EGFP)Ems/Mmjax
Stock Number:
032920-JAX
Citation ID:
RRID:MMRRC_032920-JAX
Major Collection:
Pleiades Promoter Project

Strain Information

Gene Details

Hprt1tm8(Ple151-EGFP)Ems
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 8, Elizabeth M Simpson
Synonyms: Hprt1tm8(Ple151-EGFP;mEMS1615)Ems, Hprt1tm8(OLIG1-EGFP)Ems, Ple151;OLIG1-D;mEMS1615, Hprt1tm8(mEMS1615)Ems
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Targeted Mutation
EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Targeted Mutation
Hprt1
Name: hypoxanthine phosphoribosyltransferase 1
Synonyms: Hprt1, Hprt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 15452
HGNC: HGNC:5157
Homologene: 56590

Additional Resources
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OLIG1
Name: oligodendrocyte transcription factor 1
Type: Gene
Species: Homo sapiens (human)
Chromosome: 21
Alteration at locus: Targeted Mutation
Genetic Alterations
The Ple151-EGFP transgene (pEMS1172) was designed with a Ple151 MiniPromoter ( a combination of four regulatory sequences from the human oligodendrocyte transcription factor 1 (OLIG1) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1204 embryonic stem cells (generated from F1 generation male pups born of C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males.
Genotype Determination
ES Cell Line
mEMS1204 derived from (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1

Strain Description

Phenotype

Homozygous: Not evaluated

Heterozygous: These Ple151-EGFP;mEMS1615 mice have the Ple151-EGFP transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human oligodendrocyte transcription factor 1 (OLIG1) gene directing expression of enhanced green fluorescent protein (EGFP). These Ple151-EGFP;mEMS1615 mice may be useful in studying OLIG1-expressing cells in the brain and diseases affecting the brain.

The donating investigator reports: Positive EGFP immunoreactivity was detected across numerous brain regions. EGFP-positive cells show darker staining in the nucleus with a lighter staining halo of processes. In the brainstem, cortex and thalamus, numerous immunopositive profiles are detected while fewer profiles are seen in the olfactory bulb, striatum and cerebellum. In contrast to the predicted expression of OLIG1, the EGFP expression does not appear to be enriched in white matter tracts such as the corpus callosum.

MeSH Terms
  • Animals
  • Brain/metabolism
  • Cell Differentiation/genetics
  • Computational Biology
  • Databases, Genetic
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Expression
  • Gene Expression Profiling/statistics & numerical data
  • Gene Knock-In Techniques
  • Genes, Reporter
  • Genomics
  • Humans
  • Mice
  • Mice, Transgenic
  • Neurons/cytology
  • Neurons/metabolism
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
Strain Development
Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS1615) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino). Female progeny (obligate HprtPle151-EGFP;mEMS1615 heterozygotes) also harboring a Tyr+ allele in combination with the Aw (agouti, white belly) and a (non-agouti/black) coat color alleles were identified and bred with C57BL/6J inbred males. Next, hemizygous HprtPle151-EGFP;mEMS1615 mice were bred with C57BL/6J inbred mice for at least three generations prior to arrival at the MMRRC. Upon arrival, these mice were bred with C57BL/6J inbred mice for at least one generation to establish the live colony.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Research Tools

Strain Origin

Donor
Elizabeth Simpson, University of British Columbia
Primary Reference
Portales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF;onaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Schmouth JF; Swanson MI; Tam B; Ticoll A; Turner JL; Varhol R; Vermeulen J; Watkins RF; Wilson G; Wong BK; Wong SH; Wong TY; Yang GS; Ypsilanti AR; Jones SJ; Holt RA; Goldowitz D; Wasserman WW; Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 2010 Sep 21;107(38):16589-94 (Medline PMID: 20807748)

Colony and Husbandry Information

Special Considerations

This targeted mutation is on the X chromosome. The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males. Note, homozygous females may also be bred with hemizygous males (as long as there are no reported problems with such a breeding scheme).

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
032920-JAX-SPERM Cryo-preserved spermatozoa $459.00 / Non-Profit Aliquot Approximate quantity3
032920-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.