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Homozygous: Not evaluated
Heterozygous: These Ple5-lacZ;mEMS4645 mice have the Ple5-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the Angiomotin like 1 (AMOTL1) gene directing expression of β-galactosidase (lacZ). These Ple5-lacZ;mEMS4645 mice may be useful in studying AMOTL1-expressing cells in the brain and diseases affecting the brain.
Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS4645) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino) to establish the mutant colony. The donating investigator reports that these Ple5-lacZ;mEMS4645 mutant mice were bred with C57BL/6J wild-type mice for three generations prior to sending to the MMRRC (see SNP analysis results below). Upon arrival, Ple5-lacZ;mEMS4645 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony. The rederived mice may still be segregating for the Tyrc-2J mutation.
A SNP (single nucleotide polymorphism) panel analysis covering all 19 chromosomes and the X chromosome was performed by the MMRRC on the obligate female heterozygous mice rederived from frozen sperm. This revealed 6 markers, two each on chromosomes 8, 11, and 13, that were not fixed for C57BL/6 allele-type (e.g.: still segregating for 129S allele-type markers and/or 129P allele-type markers). These data suggest the mice may have been backcrossed to C57BL/6 for 1-2 fewer generations than reported prior to arrival at the MMRRC.
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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