Strain Name:
B6.129P2(Cg)-Hprttm53(CAG-lacZ)Ems/Mmjax
Stock Number:
032963-JAX
Citation ID:
RRID:MMRRC_032963-JAX
Major Collection:

Gene Information

Hprttm53(CAG-lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 53, Elizabeth M Simpson
Synonyms: Hprttm53(mEMS3960)Ems, CAG-lacZ
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
Hprt
Name: hypoxanthine guanine phosphoribosyl transferase
Synonyms: Hprt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 15452
HGNC: HGNC:5157
Homologene: 56590
lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Alteration at locus: Targeted Mutation
Genetic Alterations
The CAG-lacZ transgene (pEMS1488) was designed with the 1627 bp CAG promoter (a human cytomegalovirus enhancer, chicken beta-actin promoter and 5' UTR, and chicken beta-actin intron/rabbit beta-globin splice acceptor, all from the pDrive-CAG vector) upstream of a frt-flanked β-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1202 embryonic stem cells (generated from F1 generation male pups [hemizygous for the Hprtb-m3 allele and wild-type at the Gt(ROSA)26Sor locus] born from C57BL/6-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] and 129S-Gt(ROSA)26Sortm1Sor heterozygous males.
Genotype Determination
ES Cell Line
mEMS1202 derived from (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Phenotype

Homozygous: Not evaluated

Heterozygous: These CAG-lacZ;mEMS3960 mice have the CAG-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the CAG promoter directing expression of β-galactosidase (lacZ). These CAG-lacZ;mEMS3960 mice have widespread expression of lacZ throughout all tested brain tissue and are a positive control for experiments using lacZ-expressing Pleiades Promoter Project lines.

MeSH Terms
  • Animals
  • Brain/metabolism
  • Cell Differentiation/genetics
  • Computational Biology
  • Databases, Genetic
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Expression
  • Gene Expression Profiling/statistics & numerical data
  • Gene Knock-In Techniques
  • Genes, Reporter
  • Genomics
  • Humans
  • Mice
  • Mice, Transgenic
  • Neurons/cytology
  • Neurons/metabolism
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
Strain Development
Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS3960) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino) to establish the mutant colony. The donating investigator reports that these CAG-lacZ;mEMS3960 mutant mice were bred with C57BL/6J wild-type mice for four generations prior to sending to the MMRRC. Upon arrival, CAG-lacZ;mEMS3960 mutant mice were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony. The rederived mice may still be segregating for the Tyrc-2J mutation.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
  • Research Tools
Donor
Elizabeth Simpson, University of British Columbia
Primary Reference
Portales-Casamar E; Swanson DJ; Liu L; de Leeuw CN; Banks KG; Ho Sui SJ; Fulton DL; Ali J; Amirabbasi M; Arenillas DJ; Babyak N; Black SF;onaguro RJ; Brauer E; Candido TR; Castellarin M; Chen J; Chen Y; Cheng JC; Chopra V; Docking TR; Dreolini L; D'Souza CA; Flynn EK; Glenn R; Hatakka K; Hearty TG; Imanian B; Jiang S; Khorasan-zadeh S; Komljenovic I; Laprise S; Liao NY; Lim JS; Lithwick S; Liu F; Liu J; Lu M; McConechy M; McLeod AJ; Milisavljevic M; Mis J; O'Connor K; Palma B; Palmquist DL; Schmouth JF; Swanson MI; Tam B; Ticoll A; Turner JL; Varhol R; Vermeulen J; Watkins RF; Wilson G; Wong BK; Wong SH; Wong TY; Yang GS; Ypsilanti AR; Jones SJ; Holt RA; Goldowitz D; Wasserman WW; Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A 2010 Sep 21;107(38):16589-94 (Medline PMID: 20807748)

Colony and Husbandry Information

The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032963-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032963-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.