Strain Name:
B6;129S-Zmpste24tm1Sgy/Mmucd
Stock Number:
000033-UCD
Citation ID:
RRID:MMRRC_000033-UCD
Other Names:
Zmpste24 deficient, B6;129S-Zmpste24tm1Sgy/Mmucd

Strain Information

Zmpste24tm1Sgy
Name: zinc metallopeptidase, STE24; targeted mutation 1, Steven G Young
Synonyms: Zmeste24-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 230709
Homologene: 4277
Genetic Alterations
The exon of the zinc metalloproteinase that encodes the critical H-E-X-X-H metalloproteinase domain was replaced with a loxP site.
Genotype Determination
ES Cell Line
RF8 derived from 129S4/SvJae
Phenotype
Severe osteoporosis, bone fractures with poor bone healing. Develop fractured bones in multiple locations, most prominently of the ribs adjacent to the costovertebral joints, by age 2-3 months.
Mammalian Phenotype Terms
Allelic Composition: Zmpste24tm1Sgy/Zmpste24tm1Sgy (Genetic Background: B6.129S4-Zmpste24tm1Sgy )

Allelic Composition: Zmpste24tm1Sgy/Zmpste24tm1Sgy (Genetic Background: involves: 129S4/SvJae )

Allelic Composition: Zmpste24tm1Sgy/Zmpste24tm1Sgy (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

Allelic Composition: Zmpste24tm1Sgy/Zmpste24+ (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cell Membrane/physiology
  • Chimera
  • Cloning, Molecular
  • Conserved Sequence
  • Endopeptidases/deficiency
  • Endopeptidases/genetics
  • Endopeptidases/metabolism
  • Gene Library
  • Genetic Vectors
  • Humans
  • Liver/metabolism
  • Mating Factor
  • Membrane Proteins/deficiency
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Metalloendopeptidases/deficiency
  • Metalloendopeptidases/genetics
  • Metalloendopeptidases/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Peptides/genetics
  • Pheromones/genetics
  • Proprotein Convertases
  • Protein Prenylation
  • Protein Processing, Post-Translational
  • Recombinant Proteins/metabolism
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
Strain Development
C57BL/6 and 129 mixture. Maintained by sibling inbreeding prior to transfer to MMRRC. Since transfer to MMRRC this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6.129S-Zmpste24tm1Sgy/Mmcd (see MMRRC #15958), that is 99% C57BL/6J by micro satellite testing.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Mouse model of osteoporosis
Donor
Stephen G. Youngm Ph.D., Gladstone Institue of Cardiovascular Disease, University of California, San Francisco
Primary Reference
Leung GK, Schmidt WK, Bergo MO, Gavino B, Wong DH, Tam A, Ashby MN, Michaelis S, Young SG. Biochemical studies of Zmpste24-deficient mice. J Biol Chem. 2001 Aug 3;276(31):29051-8. (Medline PMID: 11399759)

Colony and Husbandry Information

Homozygotes need to be mated at reproductive maturity due to early onset of bone disease.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Wild-type Female x Heterozygous Male
  • Heterozygous Female x Wild-type Male
  • Heterozygous Female x Heterozygous Male
Generation
Unknown
Overall Breeding Performance
Excellent (heterozygotes)
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
10
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000033-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000033-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000033-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.