Strain Detail Sheet

Strain Name    :

129S1.129-Nr0b1tm1Lja/Mmmh

Stock Number :

033004-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Nr0b1tm1Lja
Name: targeted mutation 1, J Larry Jameson
Alteration at locus: Targeted Mutation
Gene Symbol: Nr0b1
Name: nuclear receptor subfamily 0, group B, member 1
Chromosome: X
Alteration at locus: Targeted Mutation

Genetic Alterations:
AhCh(Dax1) was isolated from a mouse genomic DNA library, mapped and sequenced. The targeting vector contains 5.5kb of 5' and 3.1kb of 3' gene sequences. A single loxP site was inserted upstream of the splice acceptor signal of exon 2. The phosphoglycerate kinase(PGK) promotor-driven, floxed neomycon cassette was inserted downsteam of exon 2 and PGK promotor-driven thymidine kinase (TK) gene cassette was placed upstream of exon 1.

Genotype Determination:

ES Cell Line: R1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: None.

Heterozygouss phenotype: None.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Nr0b1tm1Lja/-
        C57BL/6JEi
  • reproductive system phenotype
    • abnormal primary sex determination (MGI Ref ID J:98922)
      • primary sex reversal (MGI Ref ID J:98922)
        • complete male to female sex-reversal is observed
        • QTLs from B6 Chr4, Chr1, and a genetic interaction between Chr1 and Chr8 are implicated in the sex reversal
Nr0b1tm1Lja/-
        involves: C57BL/6JEi * DBA/2J
  • reproductive system phenotype
    • abnormal primary sex determination (MGI Ref ID J:98922)
      • In the presence of a Mus domesticus poschiavinus Y chromosome, this background produces XY mice with the following gonad development: 13.8% have testes; 70.7% contain both ovarian and testicular tissue and 15.5% are completely sex-reversed.
      • ovotestis (MGI Ref ID J:98922)
        • Partial sex reversal was seen with 70.7% of the hemizygotes having at least one ovotestis.
      • primary sex reversal (MGI Ref ID J:98922)
        • On this background 15.5% of the hemizygotes show male to female sex reversal.

Strain of Origin: 129, B6 mix

Strain genetic background: 129S1/SvImJ

Strain Development: The strain was developed by insertion of a targeted mutation into ES cells which were then injected to make chimeras.Chimeras were backcrossed to 129X1/SvJ to generate targeted founder animals in 1998. From 1998-2001 the strain was intercrossed and backcrossed to 129S1/SvImJ. In 2005 the strain was submitted for speed congenics with Charles River and is now congenic on 129S1/SvImJ. It is maintained by sib/sib intercrosses interspersed with periodic backcrosses to 129S1/SvImJ.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Endocrine Deficiency
  • Models for Human Disease
  • Reproduction
  • Research Tools

Strain Origin

Donor: J. Larry Jameson, MD. PhD, Northwestern University

Primary Reference:

  • Yu RN; Ito M; Saunders TL; Camper SA; Jameson JL, Role of Ahch in gonadal development and gametogenesis [see comments], Nat Genet 1998 Dec;20(4):353-7. (Medline PMID: 9843206)
  • Jeffs B; Meeks JJ; Ito M; Martinson FA; Matzuk MM; Jameson JL; Russell LD, Blockage of the rete testis and efferent ductules by ectopic sertoli and leydig cells causes infertility in dax1-deficient male mice., Endocrinology 2001 Oct;142(10):4486-95. (Medline PMID: 11564714)
  • Jeffs B; Ito M; Yu RN; Martinson FA; Wang ZJ; Doglio LT; Jameson JL, Sertoli cell-specific rescue of fertility, but not testicular pathology, in Dax1 (Ahch)-deficient male mice., Endocrinology 2001 Jun;142(6):2481-8. (Medline PMID: 11356697)

Colony and Husbandry Information

Special Considerations

Sib-mating.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
033004-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
033004-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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