Strain Name:
C3Fe.B6-Zbtb167t/Mmjax
Stock Number:
033008-JAX
Citation ID:
RRID:MMRRC_033008-JAX

Gene Information

Zbtb167t
Name: zinc finger and BTB domain containing 16; seven toes
Synonyms: Plzf7t, 7t
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Chemically Induced
Zbtb16
Name: zinc finger and BTB domain containing 16
Synonyms: PLZF, Green's luxoid, Zfp145
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235320
VEGA: 9
Homologene: 21214
Genetic Alterations
An A to G transition at nucleotide 379 of the Zbtb16 cDNA (NM_001033324). This is predicted to alter amino acid 44 from the polar and acidic amino acid glutamic acid, into the nonpolar neutral amino acid glycine (Glu44Gly).
Genotype Determination
Phenotype
Homozygous: Mice that are homozygous for the mutation are viable and fertile. Mice exhibit multiple limb patterning defects, but not the germ cell depletion phenotype observed in other Zbtb16 alleles. Hindlimb polydactyly exists in 100% of mice however, other skeletal deformities exhibit varying degrees of penetrance including hindpaw webbing (20%), metatarsal fusions (13%), failure of the tibia and fibia to fuse (47%), thickened fibulae (40%), absence of the fibula (33%), elongated 8th and 9th ribs (73%) and malformed xiphoid processes (40%). The donating investigator indicates that homozygous mice weigh approximately 17% less than controls. This mutant mouse strain may be useful in studies of skeletal development and germ cell lineage.

Heterozygous: Less than 3% of heterozygotes exhibit the hindlimb polydactyly phenotype.
Mammalian Phenotype Terms
Allelic Composition: Zbtb167t/Zbtb167t (Genetic Background: involves: C3HeB/FeJ * C57BL/6J )

MeSH Terms
  • Alleles
  • Animals
  • Body Patterning/genetics
  • Bone and Bones/abnormalities
  • Infertility, Male/genetics
  • Kruppel-Like Transcription Factors/genetics
  • Kruppel-Like Transcription Factors/physiology
  • Male
  • Mice
  • Mutation, Missense
  • Point Mutation
  • Promyelocytic Leukemia Zinc Finger Protein
  • Skeleton
  • Spermatozoa/physiology
Strain Development
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C3HeB/FeJ females. The mutation results in an A to G change at nucleotide 379 altering the corresponding amino acid from glutamic acid to glycine at position 44. This strain has been backcrossed to C3HeB/FeJ for a minimum 6 of generations. Upon arrival, mice were bred to C3HeB/FeJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Reproduction
Donor
John C. Schimenti, Ph.D., Cornell University
Primary Reference
Ching YH; Wilson LA; Schimenti JC, An allele separating skeletal patterning and spermatogonial renewal functions of PLZF., BMC Dev Biol. 2010 Mar 25;10:33. (Medline PMID: 20338044)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
033008-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
033008-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.