Strain Name:
129P2/OlaHsd-Apobec3Gt(XN450)Byg/Mmucd
Stock Number:
033009-UCD
Citation ID:
RRID:MMRRC_033009-UCD
Other Names:
XN450

Strain Information

Apobec3Gt(XN450)Byg
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3; gene trap XN450, BayGenomics
Synonyms: Apobec3-, mA3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Gene Trap
Apobec3
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3
Synonyms: CEM15, Rfv3, Rfv-3, Gm20117
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Gene Trap
NCBI: 80287
Homologene: 105420
Genetic Alterations
An 8 kb beta-Geo cassette gene trap was inserted between exons 4 and 5 of Apobec3.
Genotype Determination

ES Cell Line
ES Cell line XN450 (MMRRC:009705) from parental cell line E14Tg2a.4 derived from 129P2/OlaHsd.
Phenotype
Homozygous: No Physical defects, but more susceptible retrovirus infection.

Heterozygous: Normal.
Mammalian Phenotype Terms
Allelic Composition: Apobec3Gt(XN450)Byg/Apobec3Gt(XN450)Byg (Genetic Background: 129P2/OlaHsd-Apobec3Gt(XN450)Byg )

Allelic Composition: Apobec3Gt(XN450)Byg/Apobec3Gt(XN450)Byg (Genetic Background: B6.129P2-Apobec3Gt(XN450)Byg )

Allelic Composition: Apobec3Gt(XN450)Byg/Apobec3Gt(XN450)Byg (Genetic Background: B6.Cg-Apobec3Gt(XN450)Byg )

MeSH Terms
  • Animals
  • Antibodies, Viral/biosynthesis
  • Antibodies, Viral/blood
  • Antibodies, Viral/immunology
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytidine Deaminase/genetics
  • Cytidine Deaminase/metabolism
  • Friend murine leukemia virus/immunology
  • Friend murine leukemia virus/physiology
  • Immunity, Innate
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Neutralization Tests
  • Retroviridae Infections/genetics
  • Retroviridae Infections/immunology
  • Retroviridae Infections/virology
  • Tumor Virus Infections/genetics
  • Tumor Virus Infections/immunology
  • Tumor Virus Infections/virology
  • Viremia
Strain Development
ES cell line XN450 was implanted into pseudopregnant mice, and chimeras were crossed with 129P2/OlaHsd mice once
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Virology
Donor
Warner Greene, M.D., Ph.D., Gladstone Institute of Virology and Immunology.
Primary Reference
Santiago ML; Montano M; Benitez R; Messer RJ; Yonemoto W; Chesebro B; Hasenkrug KJ; Greene WC, Apobec3 encodes Rfv3, a gene influencing neutralizing antibody control of retrovirus infection., Science 2008 Sep 5;321(5894):1343-6. (Medline PMID: 18772436)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Dilute
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
10
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
033009-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
033009-UCD-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.