Strain Detail Sheet

Strain Name:
C57BL/6J-Cd40lgm1BtlrMmucd
Stock Number:
033011-UCD
Citation ID:
RRID:MMRRC_033011-UCD
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Cd40lgm1Btlr
Name: CD40 ligand; mutation 1, Bruce Beutler
Synonyms: walla
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Chemically Induced
Cd40lg
Name: CD40 ligand
Synonyms: Tnfsf5, T-BAM, HIGM1, CD154, IMD3, gp39, Ly-62, Cd40L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 21947
Homologene: 56
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Genetic Alterations
ENU-induced T to C transition at position 673 of the Cd40lg transcript (NM_011616.2 from NCBI Build 37.1), in exon 5 of 5 total exons. The mutation results in substitution of serine with proline at position 221 of the CD40L protein, which is located in the TNF-like extracellular region.
Genotype Determination

Strain Description

Phenotype
Homozygous: Homozygous walla mice exhibit absent T-dependent IgG responses to the recombinant Semliki Forest Virus (rSFV)-encoded model antigen ?-galactosidase. They display normal T-independent IgM responses and serum IgA levels. The frequencies and surface phenotypes of peripheral blood T cells and B cells are normal in walla homozygotes.

Heterozygous: Wild type.
Mammalian Phenotype Terms
Allelic Composition: Cd40lgm1Btlr/Cd40lgm1Btlr (Genetic Background: C57BL/6J-Cd40lgm1Btlr )
Allelic Composition: Cd40lgm1Btlr/Cd40lgm1Btlr (Genetic Background: involves: C57BL/6J )
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology

Strain Origin

Donor
Bruce Beutler, M.D. The Scripps Research Institute
Primary Reference
Arnold CN, Pirie E, Moresco EMY, Beutler B. Record for walla, updated 26 July 2010. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations

Random intra-strain mating.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.