Strain Detail Sheet

Strain Name    :

B6.C(129)-Traf4m1Pav/Mmmh

Stock Number :

033049-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Traf4m1Pav
Name: mutation 1, William J Pavan
Alteration at locus: Chemically Induced
Gene Symbol: Traf4
Name: TNF receptor associated factor 4
Chromosome: 11
Alteration at locus: Chemically Induced

Genetic Alterations:
C to T mutation in the 5' splice site of intron 2 that results in skipping of exon 2. The donor has detected mRNA transcript and predicts it would produce truncated or altered protein.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Incompletely penetrant homozygous lethality. E11.5 embryos have ectopic clusters of cells at lower dorsal neural tube. Liveborn are runted, sickly, with craniofacial and eye dysmorphology and hydrocephaly. Liveborn dead by 4 weeks.

Heterozygous phenotype: No mutant phenotype observed. Normal growth and reproduction.

Strain of Origin: BALB/cJ

Strain genetic background: C57BL/6, BALB/c mix

Strain Development: BALB/c male was ENU mutagenized and mated to C57BL/6 Sox10-lacz/+ female. Embryos showed lacz+ ectopic cell clusters at E11.5 at lower dorsal neural tube. ENU mutation was positionally cloned and identified. Heterozygote carriers were mated to C57BL/6J wildtype or C57BL-Sox10-lacz mice, for 5-10 generations.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology

Strain Origin

Donor: Bill Pavan, Ph.D., NHGRI/NIH

Primary Reference:

Buac K; Watkins-Chow DE; Loftus SK; Larson DM; Incao A; Gibney G; Pavan WJ, A Sox10 expression screen identifies an amino acid essential for Erbb3 function., PLoS Genet 2008 Sep;4(9):e1000177 (Medline PMID: 18773073)

Colony and Husbandry Information

Special Considerations

Liveborn homozygotes need to be weaned late and then weaned to mush because they are runted and sickly. Normal mouse colony husbandry for heterozygotes.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
033049-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
033049-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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