Strain Name:
B6.C(129)-Traf4m1Pav/Mmmh
Stock Number:
033049-MU
Citation ID:
RRID:MMRRC_033049-MU

Strain Information

Traf4m1Pav
Name: TNF receptor associated factor 4; mutation 1, William J Pavan
Synonyms: modifier of Sox10 expression pattern 2, Traf4msp2, msp2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Traf4
Name: TNF receptor associated factor 4
Synonyms: msp2, A530032M13Rik, CART1
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 22032
Homologene: 3173
Genetic Alterations
C to T mutation in the 5' splice site of intron 2 that results in skipping of exon 2. The donor has detected mRNA transcript and predicts it would produce truncated or altered protein.
Genotype Determination
Phenotype
Homozygous: Incompletely penetrant homozygous lethality. E11.5 embryos have ectopic clusters of cells at lower dorsal neural tube. Liveborn are runted, sickly, with craniofacial and eye dysmorphology and hydrocephaly. Liveborn dead by 4 weeks.

Heterozygous: No mutant phenotype observed. Normal growth and reproduction.
MeSH Terms
  • Alleles
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Amino Acids/genetics
  • Amino Acids/metabolism
  • Animals
  • Base Sequence
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Ethylnitrosourea/pharmacology
  • Gene Expression
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Neural Crest/metabolism
  • Phenotype
  • Phosphorylation
  • RNA, Messenger/metabolism
  • Receptor, ErbB-3/genetics
  • Receptor, ErbB-3/metabolism
  • SOXE Transcription Factors
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
Strain Development
BALB/c male was ENU mutagenized and mated to C57BL/6 Sox10-lacz/+ female. Embryos showed lacz+ ectopic cell clusters at E11.5 at lower dorsal neural tube. ENU mutation was positionally cloned and identified. Heterozygote carriers were mated to C57BL/6J wildtype or C57BL-Sox10-lacz mice, for 5-10 generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
Donor
Bill Pavan, Ph.D., National Human Genome Research Institute/National Institutes of Health
Primary Reference
Buac K; Watkins-Chow DE; Loftus SK; Larson DM; Incao A; Gibney G; Pavan WJ, A Sox10 expression screen identifies an amino acid essential for Erbb3 function., PLoS Genet. 2008 Sep;4(9):e1000177 (Medline PMID: 18773073)

Colony and Husbandry Information

Liveborn homozygotes need to be weaned late and then weaned to mush because they are runted and sickly. Normal mouse colony husbandry for heterozygotes.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
033049-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
033049-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.