Strain Name:
B6.Cg-Faslgld Tcrbtm1Mom/Mmnc
Stock Number:
000336-UNC
Citation ID:
RRID:MMRRC_000336-UNC

Strain Information

Tcrbtm1Mom
Name: T cell receptor beta chain; targeted mutation 1, Peter Mombaerts
Synonyms: TCR-Cbeta-, TCRbeta-, TCRb-, beta-, CBeta-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Tcrb
Name: T cell receptor beta chain
Synonyms: TCRbeta, Tib
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Faslgld
Name: Fas ligand; generalized lymphoproliferative disease
Synonyms: Tnfsf6gld, gld, CD95-, FasL-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Fasl
Name: Fas ligand
Synonyms: CD178, Tnfsf6, Fas-L, APT1LG1, Fasl, CD95L
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Spontaneous Mutation
NCBI: 14103
Homologene: 533
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Mice homozygous for the Tcrbtm1Mom targeted mutation are viable and fertile. Mice are deficient in the alpha/beta T-cell receptor. The total number of cells in the thymus is ~8% that of wildtype; CD4+CD8+ cells ~6% of wildtype. Gamma/delta thymocytes undergo normal differentiation. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
    Mice homozygous for the lymphoproliferation spontaneous mutation (Faslpr) show systemic autoimmunity, massive lymphadenopathy associated with proliferation of aberrant T cells, arthritis, and immune complex glomerulonephrosis. These mice are useful as sources of alpha/beta T cell-free gamma/delta T cells that cannot express Fas ligand. They are useful in defining the role of Fas ligand in gamma/delta T cell function.
    Mammalian Phenotype Terms
    Allelic Composition: Tcrbtm1Mom/Tcrbtm1Mom (Genetic Background: B6;129P2-Tcrbtm1Mom/J )

    Allelic Composition: Tcrbtm1Mom/Tcrbtm1Mom (Genetic Background: involves: 129P2/OlaHsd * BALB/c )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: B6.C3-Faslgld )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: B6Smn.C3-Faslgld/J )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: C3H/HeJ-Faslgld )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: C3H/HeJ-Faslgld/J )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: involves: C3H/HeJ )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: involves: C3H/HeJ * C57BL/6 )

    Allelic Composition: Faslgld/Faslgld (Genetic Background: involves: C3H/HeJ * CBA )

    MeSH Terms
    • Age Factors
    • Animals
    • Antibodies/physiology
    • Antigens, CD/biosynthesis
    • Apoptosis/immunology
    • Autoimmune Diseases/immunology
    • Autoimmune Diseases/metabolism
    • B-Lymphocytes/immunology
    • B7-1 Antigen/biosynthesis
    • B7-2 Antigen
    • Bone Marrow Cells
    • Immunoglobulin D/biosynthesis
    • Immunoglobulin M/biosynthesis
    • Lymphocyte Activation
    • Lymphocyte Subsets/immunology
    • Lymphocyte Subsets/metabolism
    • Lymphoproliferative Disorders/immunology
    • Lymphoproliferative Disorders/metabolism
    • Membrane Glycoproteins/biosynthesis
    • Mice
    • Mice, Inbred C57BL
    • Peritoneal Cavity/cytology
    • Spleen/cytology
    • Up-Regulation/immunology
    • fas Receptor/biosynthesis
    • fas Receptor/immunology
    • Apoptosis
    • Arthritis, Infectious/immunology
    • Borrelia burgdorferi Group/immunology
    • CD4-Positive T-Lymphocytes/immunology
    • CD4-Positive T-Lymphocytes/microbiology
    • CD8-Positive T-Lymphocytes/immunology
    • Clone Cells
    • DNA Primers
    • Flow Cytometry
    • Humans
    • Immunophenotyping
    • Lyme Disease/immunology
    • Polymerase Chain Reaction
    • Receptors, Antigen, T-Cell, gamma-delta/analysis
    • Receptors, Antigen, T-Cell, gamma-delta/biosynthesis
    • Receptors, Antigen, T-Cell, gamma-delta/immunology
    • Synovial Fluid/immunology
    • T-Lymphocyte Subsets/immunology
    • fas Receptor
    • Cells, Cultured
    • Fas Ligand Protein
    • Kinetics
    • Listeriosis/immunology
    • Membrane Glycoproteins/physiology
    • Mice, Inbred C3H
    • Receptors, Antigen, T-Cell/immunology
    • Spleen/immunology
    • T-Lymphocytes/cytology
    • T-Lymphocytes/immunology
    • fas Receptor/physiology
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Immunology and Inflammation
    Donor
    Rebecca L. O'Brien, Ph.D., National Jewish Medical and Research Center
    Primary Reference
    • Weintraub JP, Eisenberg RA, Cohen PL. Up-regulation of Fas and the costimulatory molecules B7-1 and B7-2 on peripheral lymphocytes in autoimmune B6/gld mice. J Immunol. 1997 Oct 15;159(8):4117-26. (Medline PMID: 9379003)
    • Vincent MS, Roessner K, Lynch D, Wilson D, Cooper SM, Tschopp J, Sigal LH, Budd RC. Apoptosis of Fashigh CD4+ synovial T cells by borrelia-reactive Fas-ligand(high) gamma delta T cells in Lyme arthritis. J Exp Med. 1996 Dec 1;184(6):2109-17. (Medline PMID: 8976167)
    • Mukasa A, Lahn M, Fleming S, Freiberg B, Pflum E, Vollmer M, Kupfer A, O'Brien R, Born W. Extensive and preferential Fas/Fas ligand-dependent death of gammadelta T cells following infection with Listeria monocytogenes. Scand J Immunol. 2002 Sep;56(3):233-47. (Medline PMID: 12193224)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000336-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.