Strain Detail Sheet

Strain Name    :

B6.Cg-Faslgld Tcrbtm1Mom/Mmnc

Stock Number :

000336-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tcrbtm1Mom
Name: targeted mutation 1, Peter Mombaerts
Alteration at locus: Knockout
Gene Symbol: Tcrb
Name: T cell receptor beta chain
Chromosome: 6
Alteration at locus: Knockout
Allele Symbol: Faslgld
Name: generalized lymphoproliferative disease
Alteration at locus: Spontaneous Mutation
Gene Symbol: Fasl
Name: Fas ligand (TNF superfamily, member 6)
Chromosome: 1
Alteration at locus: Spontaneous Mutation

Genetic Alterations:

Genotype Determination:

  • Donor's Protocol
  • Center protocol and contact for technical support will be shipped with mice.

ES Cell Line: E14

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Mice homozygous for the Tcrbtm1Mom targeted mutation are viable and fertile. Mice are deficient in the alpha/beta T-cell receptor. The total number of cells in the thymus is ~8% that of wildtype; CD4+CD8+ cells ~6% of wildtype. Gamma/delta thymocytes undergo normal differentiation. Mice may develop inflammatory bowel disease beginning at ~4-6 months of age.
Mice homozygous for the lymphoproliferation spontaneous mutation (Faslpr) show systemic autoimmunity, massive lymphadenopathy associated with proliferation of aberrant T cells, arthritis, and immune complex glomerulonephrosis. These mice are useful as sources of alpha/beta T cell-free gamma/delta T cells that cannot express Fas ligand. They are useful in defining the role of Fas ligand in gamma/delta T cell function.

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Research Applications

  • Cancer
  • Immunology and Inflammation

Strain Origin

Donor: Rebecca L. O'Brien, Ph.D., National Jewish Medical and Research Center

Primary Reference:

  • Weintraub JP, Eisenberg RA, Cohen PL. Up-regulation of Fas and the costimulatory molecules B7-1 and B7-2 on peripheral lymphocytes in autoimmune B6/gld mice. J Immunol. 1997 Oct 15;159(8):4117-26. (Medline PMID: 9379003)
  • Vincent MS, Roessner K, Lynch D, Wilson D, Cooper SM, Tschopp J, Sigal LH, Budd RC. Apoptosis of Fashigh CD4+ synovial T cells by borrelia-reactive Fas-ligand(high) gamma delta T cells in Lyme arthritis. J Exp Med. 1996 Dec 1;184(6):2109-17. (Medline PMID: 8976167)
  • Mukasa A, Lahn M, Fleming S, Freiberg B, Pflum E, Vollmer M, Kupfer A, O'Brien R, Born W. Extensive and preferential Fas/Fas ligand-dependent death of gammadelta T cells following infection with Listeria monocytogenes. Scand J Immunol. 2002 Sep;56(3):233-47. (Medline PMID: 12193224 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000336-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/23/2014