Strain Name:
B6.129-Sox3tm1Lja/Mmmh
Stock Number:
034257-MU
Citation ID:
RRID:MMRRC_034257-MU

Strain Information

Sox3tm1Lja
Name: SRY (sex determining region Y)-box 3; targeted mutation 1, J Larry Jameson
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
Sox3
Name: SRY (sex determining region Y)-box 3
Synonyms: Sox-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Conditional
NCBI: 20675
Homologene: 4118
Genetic Alterations
A 16-kb genomic clone containing the entire mouse Sox3 gene was isolated from a 129/SvJ genomic library (Stratagene, La Jolla, Calif.). The targeting vector was assembled by standard recombinant techniques. A 5-kb EcoRI fragment containing the entire Sox3 gene and a 3.7-kb EcoRI fragment immediately downstream of it were cloned separately into pGEM-3z (Promega, Madison, Wis.). An XhoI/MluI fragment containing the 5' untranslated region (UTR) was subcloned from the 5-kb clone by overlapping PCR with primers containing LoxP sequences to introduce a LoxP site 153 bp upstream of the ATG translational start codon. The resulting XhoI/MluILoxP fragment was then inserted into the 5-kb clone in place of the original sequence. A second LoxP site was inserted upstream of a neomycin resistance (Neo) cassette (48). The NeoLoxP fragment was inserted into the 5-kb Sox3 clone at a HindIII site downstream of two putative poly(A) signals. The 3.7-kb genomic Sox3 clone was inserted downstream of the Neo cassette, and a thymidine kinase cassette was inserted at the 3' end of the entire construct. The final construct contains 1.6 kb of 5'-flanking sequence and 3.8 kb of 3'-flanking sequence followed by a thymidine kinase cassette. The Sox3 gene is X-linked.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Mice homozygous for the targeted mutation are viable, fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in gonads and brain results in variable growth and abnormal tooth development. Females exhibit some dead or malformed oocytes, ovaries containing small atretic follicles and impaired fertility. Males exhibit reduced testis weight, Sertoli cell vacuolization, loss of germ cells, malformed seminiferous tubules and low sperm count. This mutant strain is useful in studies of tooth development and gametogenesis.

Heterozygous/Hemizygous: No observed phenotype.
Mammalian Phenotype Terms
Allelic Composition: Sox3tm1Lja/Sox3+ (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology
  • Female
  • Gene Targeting
  • Growth Disorders/genetics
  • Growth Hormone/genetics
  • High Mobility Group Proteins/deficiency
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/physiology
  • Immunohistochemistry
  • Infertility, Female/genetics
  • Male
  • Mice
  • Mice, Knockout
  • Oogenesis/genetics
  • Ovary/abnormalities
  • Ovary/physiology
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • SOXB1 Transcription Factors
  • Sex Determination Processes
  • Spermatogenesis/genetics
  • Testis/abnormalities
  • Testis/physiology
  • Tooth Abnormalities/genetics
  • Transcription Factors
  • X Chromosome/genetics
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA Primers
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Organ Size
  • Pituitary Gland/anatomy & histology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sperm Count
  • Spermatogenesis/physiology
  • Spermatogonia/cytology
  • Spermatogonia/physiology
  • Testis/anatomy & histology
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
Strain Development
Targeted Mutagenesis Facility used two correctly targeted clones for injection into 129/SvJ blastocysts. Resulting chimeric male mice were bred with wild-type (WT) 129/SvJ animals, and progeny were screened by PCR for germ line transmission of the targeted allele. In 2003 one remaining targeted line was backcrossed onto C57BL/6 and was continually backcrossed to C57BL/6 until targeted males and females were intercrossed to produced targeted animals for cryopreservation.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Models for Human Disease
  • Neurobiology
  • Reproduction
Donor
J. Larry Jameson, M.D., Ph.D, Northwestern University
Primary Reference
  • Weiss J; Meeks JJ; Hurley L; Raverot G; Frassetto A; Jameson JL, Sox3 is required for gonadal function, but not sex determination, in males and females., Mol Cell Biol 2003 Nov;23(22):8084-91 (Medline PMID: 14585968)
  • Raverot G; Weiss J; Park SY; Hurley L; Jameson JL, Sox3 expression in undifferentiated spermatogonia is required for the progression of spermatogenesis., Dev Biol 2005 Jul 1;283(1):215-25 (Medline PMID: 15893302)

Colony and Husbandry Information

Normally, Sox3tm1Lja males are mated to C57BL/6 females: 1 male x 2 females. Litters of 6-8 pups are produced.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034257-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034257-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.