Strain Name:
FVB.Cg-Atp2a3tm1Ges/Mmjax
Stock Number:
034260-JAX
Citation ID:
RRID:MMRRC_034260-JAX

Strain Information

Atp2a3tm1Ges
Name: ATPase, Ca++ transporting, ubiquitous; targeted mutation 1, Gary E Shull
Synonyms: SERCA3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Targeted Mutation
Atp2a3
Name: ATPase, Ca++ transporting, ubiquitous
Synonyms: Serca3
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Targeted Mutation
NCBI: 53313
HGNC: HGNC:813
Homologene: 69131
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 5 of the ATPase, Ca++ transporting, ubiquitous (Atp2a3 or Serca3) gene, abolishing gene function. Serca3 is a Sarco(endo)plasmic reticulum Ca2+ ATPase which is expressed in endothelial cells, in epithelial cells of trachea, intestine, and salivary glands, and in platelets, mast cells, and lymphocytes. In these mice, acetylecholine (Ach)-induced aortic relaxation is reduced, and Ach-responsive Ca2+ stores, along with intracellular Ca2+ signaling, are diminished. These Serca3-/-mice do, however, exhibit normal basal blood pressure, glucose tolerance, and insulin secretion. These mice may be useful for studying endothelial Ca2+ signaling and aortic smooth muscle relaxation/contraction.
Heterozygous: Mice are viable, fertile, and normal in size. They exhibit normal growth, and are phenotypically similar to wildtype mice
Mammalian Phenotype Terms
Allelic Composition: Atp2a3tm1Ges/Atp2a3tm1Ges (Genetic Background: involves: Black Swiss )

MeSH Terms
  • Acetylcholine/pharmacology
  • Animals
  • Aorta/physiology
  • Blood Pressure/drug effects
  • Calcium/physiology
  • Calcium-Transporting ATPases/physiology
  • Endothelium, Vascular/enzymology
  • Endothelium, Vascular/physiology
  • In Vitro Techniques
  • Mice
  • Mice, Knockout
  • Muscle Relaxation
  • Muscle, Smooth, Vascular/physiology
  • Nitric Oxide Synthase/metabolism
  • Nitroprusside/pharmacology
  • RNA, Messenger/genetics
  • Rats
  • Sarcoplasmic Reticulum/enzymology
  • Sarcoplasmic Reticulum/physiology
  • Signal Transduction
  • Tissue Distribution
  • Vasodilation/drug effects
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 5 of the ATPase, Ca++ transporting, ubiquitous (Atp2a3 or Serca3) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to Black Swiss females, and these mice were subsequently backcrossed to FVB/N mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cardiovascular
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Liu LH; Paul RJ; Sutliff RL; Miller ML; Lorenz JN; Pun RY; Duffy JJ; Doetschman T; Kimura Y; MacLennan DH; Hoying JB; Shull GE, Defective endothelium-dependent relaxation of vascular smooth muscle and endothelial cell Ca2+ signaling in mice lacking sarco(endo)plasmic reticulum Ca2+-ATPase isoform 3.,J Biol Chem 1997 Nov 28;(48): 30538-45 (Medline PMID: 9374548)

Colony and Husbandry Information

When maintaining a live colony, homozygous mice may be bred together.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034260-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034260-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.