Strain Name:
STOCK Atp2b2tm1Ges/Mmjax
Stock Number:
034261-JAX
Citation ID:
RRID:MMRRC_034261-JAX

Gene Information

Atp2b2tm1Ges
Name: ATPase, Ca++ transporting, plasma membrane 2; targeted mutation 1, Gary E Shull
Synonyms: Pmca2 -
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: PMCA2, wms, D6Abb2e, jog, Tmy, Gena300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 19 of the ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2 or Pmca2) gene, abolishing gene function. Homozygous mice are viable with females being fertile. The expression of Pmca2 is tightly restricted to Purkinje neurons of the cerebellum, spiral ganglion nerves of the inner ear, and outer hair cells of the cochlea, and is required for both balance and hearing. Pmca2-/- mice have a decreased growth rate, an unsteady gait, difficulty in maintaining balance, and are deaf. They also exhibit an increased number of Purkinje neurons in the cerebellum, decreased thickness of the molecular layer, an absence of otoconia in the vestibular system, and abnormalities of the organ of Corti. These mice may be useful for studying the development of the vestibular and auditory systems, and the maintenance of otoconia.

Heterozygous: Mice are viable, fertile, and normal in size. They exhibit normal growth, and are phenotypically similar to wildtype mice with the exception of some hearing loss.
Mammalian Phenotype Terms
Allelic Composition: Atp2b2tm1Ges/Atp2b2tm1Ges (Genetic Background: involves: 129/Sv * 129X1/SvJ * Black Swiss )

Allelic Composition: Atp2b2tm1Ges/Atp2b2+ (Genetic Background: involves: 129/Sv * 129X1/SvJ * Black Swiss )

Allelic Composition: Atp2b2tm1Ges/Atp2b2+ (Genetic Background: involves: 129/Sv * 129X1/SvJ * Black Swiss * C3H/HeJ )

Allelic Composition: Atp2b2tm1Ges/Atp2b2+ (Genetic Background: involves: 129/Sv * 129X1/SvJ * Black Swiss * CAST/Ei )

MeSH Terms
  • Animals
  • Calcium/metabolism
  • Calcium-Transporting ATPases/genetics
  • Calcium-Transporting ATPases/physiology
  • Cation Transport Proteins
  • Cell Membrane/enzymology
  • Deafness/enzymology
  • Deafness/genetics
  • Evoked Potentials, Auditory, Brain Stem
  • Gene Targeting
  • Hair Cells, Vestibular/enzymology
  • In Situ Hybridization
  • Mice
  • Mice, Knockout
  • Otolithic Membrane/enzymology
  • Plasma Membrane Calcium-Transporting ATPases
  • Postural Balance
  • RNA, Messenger/metabolism
  • Sensation Disorders/enzymology
  • Sensation Disorders/genetics
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 19 of the ATPase, Ca++ transporting, plasma membrane 2 (Atp2b2 or Pmca2) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to Black Swiss mice. This strain was maintained on a 129/Black Swiss background. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Neurobiology
  • Sensorineural
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Kozel PJ; Friedman RA; Erway LC; Yamoah EN; Liu LH; Riddle T; Duffy JJ; Doetschman T; Miller ML; Cardell EL; Shull GE, Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2.,J Biol Chem 1998 Jul 24(273):18693-6 (Medline PMID: 9668038)

Colony and Husbandry Information

When maintaining a live colony heterozygous mice may be bred to wildtype mice from the colony. The Donating Investigator confirms that homozygous females are fertile and homozygous male fertility is unknown. Homozygous mice exhibit extreme ataxia and loss of balance.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034261-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034261-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.