Strain Name:
FVB.Cg-Slc12a2tm1Ges/Mmjax
Stock Number:
034262-JAX
Citation ID:
RRID:MMRRC_034262-JAX

Gene Information

Slc12a2tm1Ges
Name: solute carrier family 12, member 2; targeted mutation 1, Gary E Shull
Synonyms: Nkcc1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Targeted Mutation
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, mBSC2, Nkcc1, sy-ns
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Targeted Mutation
NCBI: 20496
VEGA: 18
Homologene: 20283
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 6 of the solute carrier family 12, member 2 (Slc12a2 or Nkcc1) gene, abolishing gene function. Homozygous mice are viable, fertile, and exhibit growth retardation with a 30% incidence of death by the time of weaning. Nkcc1 regulates cell volume and is expressed in vascular endothelial cells where it is required for controlling cytosolic concentrations of Cl- and K+, and in the basolateral membranes of epithelial cells where it provides an entry pathway for ions that are secreted across the apical membrane. These Nkcc1-/- mice are significantly smaller than their wildtype littermates by 1 week of age, and remain 80% smaller as adults. They are deaf, have less body fat, reduced mean arterial blood pressure, and exhibit reduced cAMP-induced short circuit currents in jejunum, cecum, and trachea. These mutant mice also demonstrate unusual head postures, and engage in circling behaviors and rapid spinning, but have difficulty maintaining their balance. The lumen of the cochlear duct of these mice is collapsed, and Reissner's membrane lies against the interdental cells of the spiral limbus, the tectorial membrane, and the stria vascularis. These mice may be useful for studying the regulation of cell volume, and the control of Cl- and K+ secretion in the maintainance of hearing and blood pressure.

Heterozygous: Mice are viable, fertile, and normal in size. They exhibit normal growth, and are phenotypically similar to wildtype mice with the exception of slightly reduced blood pressure.
Mammalian Phenotype Terms
Allelic Composition: Slc12a2tm1Ges/Slc12a2tm1Ges (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Animals, Suckling
  • Blood Pressure
  • Carrier Proteins/genetics
  • Carrier Proteins/physiology
  • Chlorides/metabolism
  • Deafness/etiology
  • Deafness/pathology
  • Digestive System/pathology
  • Epithelial Cells/metabolism
  • Genotype
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Mutant Strains
  • Potassium/metabolism
  • Sodium/metabolism
  • Sodium-Potassium-Chloride Symporters
  • Survival Rate
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 6 of the solute carrier family 12, member 2 (Slc12a2 or Nkcc1) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to Black Swiss females, and these mice were subsequently backcrossed to FVB/N mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Sensorineural
  • Cell Biology
  • Cardiovascular
  • Neurobiology
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Flagella M; Clarke LL; Miller ML; Erway LC; Giannella RA; Andringa A; Gawenis LR; Kramer J; Duffy JJ; Doetschman T; Lorenz JN; Yamoah EN; Cardell EL; Shull GE, Mice lacking the basolateral Na-K-2Cl cotransporter have impaired epithelial chloride secretion and are profoundly deaf., J Biol Chem 1999 Sep 17(274):26946-55 (Medline PMID: 10480906)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to FVB/NJ inbred mice. Homozygous mice are runted and engage in circling behavior and rapid spinning. The donating investigator confirms that 30% of homozygotes die by 4 weeks of age.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034262-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034262-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.