Strain Name:
STOCK Slc4a4tm1Ges/Mmjax
Stock Number:
034263-JAX
Citation ID:
RRID:MMRRC_034263-JAX

Gene Information

Slc4a4tm1Ges
Name: solute carrier family 4 (anion exchanger), member 4; targeted mutation 1, Gary E Shull
Synonyms: Nbc1-, NBCe1-
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Targeted Mutation
Slc4a4
Name: solute carrier family 4 (anion exchanger), member 4
Synonyms: NBC, NBC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
NCBI: 54403
Homologene: 55776
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to replace the 3' end of exon 9 of the solute carrier family 4 (anion exchanger), member 4 (Slc4a4 or Nbc1) gene with a neomycin resistance (neo) cassette. Homozygotes are viable, exhibit growth retardation, and die between 5 and 25 days of age. Nbc1 is expressed in the basolateral membrane of epithelial cells lining both the small and large intestine, in colonic crypts, as well as the kidney, eye, pancreas, and duodenum, and is required for anion secretion. Nbc1-/- mice exhibit severe metabolic and respiratory acidosis, growth retardation, reduced plasma Na+, hyperaldosteronism, splenomegaly, abnormal dentition, intestinal obstructions, and death before weaning. Intracellular pH (pHi) regulation is impaired and bioelectric colon measurements revealed increased amiloride-sensitive Na+ absorption. cAMPstimulated anion secretion is increased in proximal colon of these mutants compared to wildtype. These mice may be useful for studying pH homeostasis, bicarbonate absorption in the kidney, and intestinal and colonic anion secretion.

Heterozygous: Mice are viable, fertile, and normal in size. They exhibit normal growth, and are phenotypically similar to wildtype mice with the exception of a slight decrease in blood HCO3- levels.
Mammalian Phenotype Terms
Allelic Composition: Slc4a4tm1Ges/Slc4a4tm1Ges (Genetic Background: involves: 129S6/SvEvTac * Black Swiss )

Allelic Composition: Slc4a4tm1Ges/Slc4a4+ (Genetic Background: involves: 129S6/SvEvTac * Black Swiss )

MeSH Terms
  • Acidosis/genetics
  • Aldosterone/metabolism
  • Animals
  • Anions
  • Colon/metabolism
  • Cyclic AMP/metabolism
  • Intestines/metabolism
  • Kidney/metabolism
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Phenotype
  • Phosphorylation
  • Sodium/metabolism
  • Sodium-Bicarbonate Symporters/genetics
  • Sodium-Bicarbonate Symporters/physiology
Strain Development
A targeting vector was designed to replace the 3' end of exon 9 of the solute carrier family 4 (anion exchanger), member 4 (Slc4a4 or Nbc1) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to Black Swiss females. This strain was maintained on a 129/Black Swiss background. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Metabolism
  • Hematology
  • Immunology and Inflammation
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Gawenis LR; Bradford EM; Prasad V; Lorenz JN; Simpson JE; Clarke LL; Woo AL; Grisham C; Sanford LP; Doetschman T; Miller ML; Shull GE, Colonic anion secretory defects and metabolic acidosis in mice lacking the NBC1 Na+/HCO3- cotransporter.,2007 Mar 23(282):9042-52 (Medline PMID: 17192275)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygous animals are smaller and die between 5 and 25 days of age due to malnutrition and brittle teeth.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034263-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034263-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.