Strain Name:
FVB.Cg-Slc9a1tm1Smb/Mmjax
Stock Number:
034264-JAX
Citation ID:
RRID:MMRRC_034264-JAX

Strain Information

Slc9a1tm1Smb
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1; targeted mutation 1, Sheila M Bell
Synonyms: Nhe1-, Nhe1 -
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Targeted Mutation
Slc9a1
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
Synonyms: antiporter, Apnh, Nhe1, Nhe-1
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Targeted Mutation
NCBI: 20544
Homologene: 20660
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to insert a neomycin resistance (neo) cassette into the 3' end of exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 1 (Slc9a1 or Nhe1) gene, abolishing gene function. Nhe1 is expressed in the hippocampus, periamygdaloid cortex, and cerebellum, and is a key regulator of pH homeostasis, cell volume, and the proliferative response to growth factor stimulation. Homozygous Nhe1-/- mice are viable and indistinguishable from their littermates through the first week of age. These mice show decreased postnatal growth with ataxic gait and epileptic-like seizures at 2 weeks of age. Mortality of homozygous mutant mice is increased to 68% by 29 days of age, with a reduction to 10% under low stress conditions. Postmortem animals exhibit a waxy particulate in the ears, around the eyes and chin, and on the ventral surface of the paws. The thickness of the skin, including the epidermis, dermis, subcutaneous fat, and muscle is significantly thinner in these mutant mice. These mice also display thickening of the lamina propria and slight atrophic glandular mucosa in the stomach. These mice may be useful for studying postnatal cell proliferation and similarities between the Nhe-/- mutation and the spontaneous swe mutation within the Nhe locus.

Heterozygous: Mice are viable, fertile, and normal in size. They exhibit normal growth and are phenotypically similar to wildtype mice.
Mammalian Phenotype Terms
Allelic Composition: Slc9a1tm1Smb/Slc9a1tm1Smb (Genetic Background: involves: 129X1/SvJ * Black Swiss )

MeSH Terms
  • Amiloride/analogs & derivatives
  • Amiloride/pharmacology
  • Animals
  • Ataxia/genetics
  • Ataxia/physiopathology
  • Base Sequence
  • Body Weight
  • Chimera
  • Crosses, Genetic
  • Death
  • Embryonic and Fetal Development
  • Exons
  • Female
  • Gastric Mucosa/pathology
  • Growth Disorders/genetics
  • Growth Disorders/physiopathology
  • Homozygote
  • Lacrimal Apparatus/drug effects
  • Lacrimal Apparatus/physiology
  • Lacrimal Apparatus/physiopathology
  • Male
  • Mice
  • Mice, Knockout
  • Seizures/genetics
  • Seizures/physiopathology
  • Sodium-Hydrogen Exchangers/genetics
  • Sodium-Hydrogen Exchangers/physiology
  • Transcription, Genetic
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into the 3' end of exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 1 (Slc9a1 or Nhe1) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to Black Swiss females, and these mice were subsequently backcrossed to FVB/N mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cardiovascular
  • Neurobiology
  • Dermatology
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Bell SM; Schreiner CM; Schultheis PJ; Miller ML; Evans RL; Vorhees CV; Shull GE; Scott WJ, Targeted disruption of the murine Nhe1 locus induces ataxia, growth retardation, and seizures., Am J Physiol 1999 Apr(276):C788-95 (Medline PMID: 10199808)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or FVB/NJ inbred mice. The Donating Investigator confirms mortality rate of homozygotes is 68% by 29 days of age, with a reduction to 10% under low stress conditions.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034264-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034264-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.