Strain Name:
FVB.Cg-Slc9a2tm1Ges/Mmjax
Stock Number:
034265-JAX
Citation ID:
RRID:MMRRC_034265-JAX

Strain Information

Slc9a2tm1Ges
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2; targeted mutation 1, Gary E Shull
Synonyms: NHE2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Targeted Mutation
Slc9a2
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2
Synonyms: NHE2, 4932415O19Rik, 2210416H12Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 226999
Homologene: 20661
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 2 (Slc9a2 or Nhe2) gene, abolishing gene function. Homozygous Nhe2-/- mice are viable and normal in size, yet they produced few litters. NHE2 is expressed in gastric epithelia mucous, zymogenic, and parietal cells, as well as the in small intestine, colon, and kidney. In these mutant mice, blood pH, HCO3-, Na+ concentrations and plasma aldosterone levels are identical to wild-type levels, while net acid secretion is reduced just before weaning and is abolished in adult animals. The number of parietal and zymogenic cells of the gastric corpus are reduced. As such, parietal cells are seen in various stages of degeneration; necrotic and dead parietal cells are observed was well as some actively secreting acid, while others are immature or vacuolated. The few mature parietal cells found are filled with an increased quantity of canalicular membranes; mature zymogenic cells are rare. Mucosal thickness on the greater curvature of the stomach is decreased, the stomachs are enlarged and weigh more than in wildtype, and the lamina propria is thickened and contained numerous inflammatory cells. Nhe2-/- mice also exhibit increased Juxtaglomerular Apparatus (JGA) and renal renin content, and increased intrarenal and plasma renin activities. These mice may be useful for studying acid secretion, parietal cell viability, mucosal protection, and regulation of renal salt, water conservation, and blood pressure.

Heterozygous: mice are viable, fertile, and normal in size. They exhibit normal growth and are phenotypically similar to wildtype mice.
Mammalian Phenotype Terms
Allelic Composition: Slc9a2tm1Ges/Slc9a2tm1Ges (Genetic Background: involves: 129X1/SvJ * Black Swiss )

MeSH Terms
  • Acids/metabolism
  • Adenosine Triphosphatases/metabolism
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cell Survival/genetics
  • Cell Survival/physiology
  • Cells, Cultured
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA/analysis
  • DNA/genetics
  • DNA/metabolism
  • Female
  • Gastric Mucosa/cytology
  • Gastric Mucosa/metabolism
  • Gastrins/analysis
  • Gastrins/metabolism
  • Intestines/metabolism
  • Isomerism
  • Kidney/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred ICR
  • Mice, Knockout
  • Molecular Sequence Data
  • Mutagenesis
  • Parietal Cells, Gastric/cytology
  • Parietal Cells, Gastric/secretion
  • Parietal Cells, Gastric/ultrastructure
  • Pepsinogens/metabolism
  • Polymerase Chain Reaction
  • Potassium/metabolism
  • Pregnancy
  • Protons
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Sodium-Hydrogen Exchangers/genetics
  • Sodium-Hydrogen Exchangers/metabolism
  • Sodium-Hydrogen Exchangers/physiology
  • Stem Cells/metabolism
  • Stomach/metabolism
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 2 (Slc9a2 or Nhe2) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred to Black Swiss females, and these mice were subsequently backcrossed to FVB/N mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Metabolism
  • Internal/Organ
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Schultheis PJ; Clarke LL; Meneton P; Harline M; Boivin GP; Stemmermann G ; Duffy JJ ; Doetschman T ; Miller ML ; Shull GE, Targeted disruption of the murine Na+/H+ exchanger isoform 2 gene causes reduced viability of gastric parietal cells and loss of net acid secretion., J Clin Invest 1998 Mar 15(101):1243-53 (Medline PMID:9502765)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred together. Homozygous matings produce few litters.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034265-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034265-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.