Strain Name:
STOCK Slc9a4tm1Ges/Mmjax
Stock Number:
034266-JAX
Citation ID:
RRID:MMRRC_034266-JAX
Other Names:
Nhe4-/-

Gene Information

Slc9a4tm1Ges
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 4; targeted mutation 1, Gary E Shull
Synonyms: NHE4-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
Slc9a4
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 4
Synonyms: NHE4, D730009J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Targeted Mutation
NCBI: 110895
Homologene: 72232
Genetic Alterations
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 4 (Slc9a4) gene, abolishing gene function.  Sequence analysis showed that exon 2 and the neo gene had been excised and exon 1 was spliced to exon 3
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: Slc9a4-/- mice are viable, fertile, and normal in size.  Slc9a4 is expressed on the basolateral membrane of gastric parietal cells and zymogenic (chief) cells, and is required for normal levels of gastric acid secretion, secretory membrane development, and parietal cell and chief cell differentiation. These mutant mice display a reduction in gastric acid and their gastric mucosa contain reduced numbers of parietal cells, a loss of mature chief cells, increased numbers of mucous and undifferentiated cells, and an increase in the number of necrotic and apoptotic cells. In addition, the parietal cells of  Slc9a4-/- mice exhibit limited development of canalicular membranes and a virtual absence of tubulovesicles. These mice may be useful for studying gastric acid secretion, gastric epithelial cell differentiation, and development of secretory canalicular and tubulovesicular membranes.

Heterozygous: mice are viable, fertile, and normal in size. They exhibit normal growth and are phenotypically similar to wildtype mice.
Mammalian Phenotype Terms
Allelic Composition: Slc9a4tm1Ges/Slc9a4tm1Ges (Genetic Background: involves: 129S6/SvEvTac * Black Swiss )

MeSH Terms
  • Achlorhydria/pathology
  • Alleles
  • Alternative Splicing
  • Animals
  • Apoptosis
  • Blotting, Northern
  • Blotting, Western
  • Cell Differentiation
  • DNA, Complementary/metabolism
  • Dose-Response Relationship, Drug
  • Exons
  • Gastric Acid/metabolism
  • Gastric Acid/secretion
  • Gastrins/metabolism
  • Hydrogen-Ion Concentration
  • Immunoblotting
  • In Situ Nick-End Labeling
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron
  • Models, Biological
  • Models, Genetic
  • Mutation
  • Necrosis
  • Parietal Cells, Gastric/cytology
  • Parietal Cells, Gastric/ultrastructure
  • Phenotype
  • RNA/metabolism
  • RNA, Messenger/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium-Hydrogen Exchangers/metabolism
  • Sodium-Hydrogen Exchangers/physiology
  • Time Factors
Strain Development
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 4 (Slc9a4 or Nhe4) gene. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to Black Swiss females.
Suggested Control Mice
Wild-type littermates
  • Developmental Biology
  • Apoptosis
  • Metabolism
Donor
Gary Shull, Ph.D., University of Cincinnati
Primary Reference
Gawenis LR; Greeb JM; Prasad V; Grisham C; Sanford LP; Doetschman T; Andringa A; Miller ML; Shull GE, Impaired gastric acid secretion in mice with a targeted disruption of the NHE4 Na+/H+ exchanger. J Biol Chem 2005 Apr 1(280):12781-9 (Medline PMID: 15684419)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male
  • Homozygous female x Homozygous male
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034266-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034266-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.