Strain Name:
STOCK Slc4a2tm1Ges/Mmjax
Stock Number:
034267-JAX
Citation ID:
RRID:MMRRC_034267-JAX

Strain Information

Slc4a2tm1Ges
Name: solute carrier family 4 (anion exchanger), member 2; targeted mutation 1, Gary E Shull
Synonyms: AE2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: B3RP, Ae2
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Targeted Mutation
NCBI: 20535
Homologene: 128699
ES Cell Line
Not specified
Phenotype
Homozygous: A targeting vector was designed to replace exons 14-17 of the solute carrier family 4 (anion exchanger), member 2 (Slc9a4 or Ae2) gene with a neomycin resistance (neo) cassette, abolishing gene function. 20% of homozygous Ae2-/- mice die postnatally, with all dying by 40 days of age. Ae2 is expressed in epithelial cells of the kidney, respiratory tract, and alimentary tract. Ae2 is required for HCO3- efflux and Cl- influx across the basolateral membrane of gastric parietal cells, which are necessary for normal levels of gastric acid secretion. Ae2-/- mice are emaciated, mildly ataxic, show evidence of dehydration, exhibit hearing loss and defective development of bone, and demonstrate a failure of both molar and incisor tooth eruption.
Heterozygous phenotype: Mice are viable, fertile, and normal in size. They exhibit normal growth and are phenotypically similar to wildtype mice.
Mammalian Phenotype Terms
Allelic Composition: Slc4a2tm1Ges/Slc4a2tm1Ges (Genetic Background: involves: 129S6/SvEvTac )

Allelic Composition: Slc4a2tm1Ges/Slc4a2tm1Ges (Genetic Background: involves: 129S6/SvEvTac * Black Swiss )

MeSH Terms
  • Achlorhydria/genetics
  • Alleles
  • Animals
  • Anion Transport Proteins
  • Antiporters
  • Blotting, Northern
  • Blotting, Western
  • Cell Survival
  • Chloride-Bicarbonate Antiporters
  • Epithelial Cells/metabolism
  • Epithelium/metabolism
  • Genetic Vectors
  • Hydrogen-Ion Concentration
  • Intracellular Membranes/metabolism
  • Membrane Proteins/genetics
  • Membrane Proteins/physiology
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Microscopy, Confocal
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Mutation
  • Parietal Cells, Gastric/metabolism
  • Phenotype
  • RNA, Messenger/metabolism
  • SLC4A Proteins
  • Stomach/metabolism
  • Transgenes
Strain Development
A targeting vector was designed to replace exons 14-17 of the solute carrier family 4 (anion exchanger), member 2 (Slc9a4 or Ae2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to Black Swiss females. This strain was maintained on a 129/Black Swiss background. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
Donor
Gary Shulll, Ph.D., University of Cincinnati
Primary Reference
Gawenis LR; Ledoussal C; Judd LM; Prasad V; Alper SL; Stuart-Tilley A; Woo AL; Grisham C; Sanford LP; Doetschman T; Miller ML; Shull GE, Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric., J Biol Chem 2004 Jul 16(279):30531-9 (Medline PMID: 15123620)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygous mice have no teeth and 20% die postnatally. All homozygotes die by 40 days of age.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034267-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034267-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.