Strain Name:
B6.129(Cg)-Sox3tm1.1Lja/Mmmh
Stock Number:
034290-MU
Citation ID:
RRID:MMRRC_034290-MU

Strain Information

Sox3tm1.1Lja
Name: SRY (sex determining region Y)-box 3; targeted mutation 1, J Larry Jameson
Synonyms: Sox3-, Sox3tm1Lja
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
Sox3
Name: SRY (sex determining region Y)-box 3
Synonyms: Sox-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 20675
Homologene: 4118
Genetic Alterations
A 16-kb genomic clone containing the entire mouse Sox3 gene was isolated from a 129/SvJ genomic library (Stratagene, La Jolla, Calif.). The targeting vector was assembled by standard recombinant techniques. A 5-kb EcoRI fragment containing the entire Sox3 gene and a 3.7-kb EcoRI fragment immediately downstream of it were cloned separately into pGEM-3z (Promega, Madison, Wis.). An XhoI/MluI fragment containing the 5' untranslated region (UTR) was subcloned from the 5-kb clone by overlapping PCR with primers containing LoxP sequences to introduce a LoxP site 153 bp upstream of the ATG translational start codon. The resulting XhoI/MluILoxP fragment was then inserted into the 5-kb clone in place of the original sequence. A second LoxP site was inserted upstream of a neomycin resistance (Neo) cassette (48). The NeoLoxP fragment was inserted into the 5-kb Sox3 clone at a HindIII site downstream of two putative poly(A) signals. The 3.7-kb genomic Sox3 clone was inserted downstream of the Neo cassette, and a thymidine kinase cassette was inserted at the 3' end of the entire construct. The final construct contains 1.6 kb of 5'-flanking sequence and 3.8 kb of 3'-flanking sequence followed by a thymidine kinase cassette. A single loxP site remained in place of the single exon after cre mediated recombination of flanking loxP sites. A neo cassette included in the targeting vector was excised along with the endogenous sequence. The Sox3 gene is X-linked.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Sox3 knockout mice exhibit variable growth, abnormal tooth development, and altered function of the somatic and germ cells in both sexes.Phenotype is more severe in homozygous females, but these are difficult to create as males lose fertility early and get back some fertility later.
Mammalian Phenotype Terms
Allelic Composition: Sox3tm1.1Lja/Sox3tm1.1Lja (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology
  • Female
  • Gene Targeting
  • Growth Disorders/genetics
  • Growth Hormone/genetics
  • High Mobility Group Proteins/deficiency
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/physiology
  • Immunohistochemistry
  • Infertility, Female/genetics
  • Male
  • Mice
  • Mice, Knockout
  • Oogenesis/genetics
  • Ovary/abnormalities
  • Ovary/physiology
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • SOXB1 Transcription Factors
  • Sex Determination Processes
  • Spermatogenesis/genetics
  • Testis/abnormalities
  • Testis/physiology
  • Tooth Abnormalities/genetics
  • Transcription Factors
  • X Chromosome/genetics
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA Primers
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Organ Size
  • Pituitary Gland/anatomy & histology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sperm Count
  • Spermatogenesis/physiology
  • Spermatogonia/cytology
  • Spermatogonia/physiology
  • Testis/anatomy & histology
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
Strain Development
The Northwestern University Targeted Mutagenesis Facility used two correctly targeted clones for injection into 129/SvJ blastocysts. Resulting chimeric male mice were bred with wild-type (WT) 129S1/SvJ animals, and progeny were screened by PCR for germ line transmission of the targeted allele.The animals with the targeted allele were bred to CMV-cre animals on a 129S1/SvImJ strain backround in order to excise the LoxP cassette and create the knockout animal. Cre was eventually bred out of the line by selecting males with the excision but no cre. From 2001-2003 the excised animals were intermittantly bred with both 129X1SvJ and 129S1/SvImJ. From 2003 on the Sox2KO was backcrossed 129S1/SvImJ to be congenic on this strain backround. Simultaneously Sox3KO males of the 129S1/SvImJ strain backround were crosses with C56BL/6 females to create a congenic KO strain on C57BL/6. By the N3 generation of backcross to C57BL/6 it was discovered that Sox3KO males on the C57BL/6 backround lost early fertility and exhibited hypogonadism. At this point breeding was done through het females.The C57BL/6 strain of this model has a more severe phenotype than that observed in 129S1/SvImJ.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
  • Endocrine Deficiency
  • Models for Human Disease
  • Neurobiology
  • Reproduction
Donor
J. Larry Jameson, M.D., Ph.D., Northwestern University
Primary Reference
  • Weiss J; Meeks JJ; Hurley L; Raverot G; Frassetto A; Jameson JL, Sox3 is required for gonadal function, but not sex determination, in males and females., Mol Cell Biol. 2003 Nov;23(22):8084-91 (Medline PMID: 14585968)
  • Raverot G; Weiss J; Park SY; Hurley L; Jameson JL, Sox3 expression in undifferentiated spermatogonia is required for the progression of spermatogenesis., Dev Biol. 2005 Jul 1;283(1):215-25 (Medline PMID: 15893302)

Colony and Husbandry Information

KO males are hypogonadal and lose fertility early to to defect in germ cell development, but largely recover fertility by 12 weeks. In general we breed through females (crossed to C57BL/6 males) so that were are not holding males to age. Older males (12 weeks) do produce enough sperm for cryopreservation despite being hypogonadal.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034290-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.