Strain Detail Sheet

Strain Name    :

C57BL/6J-Irf8m1Btlr/Mmucd

Stock Number :

034294-UCD

Other Names   :

Gemini, C57BL/6J-Irf8m1Btlr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Irf8M1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Irf8
Name: interferon regulatory factor 8
Chromosome: 8
Alteration at locus: Chemically Induced

Genetic Alterations:
The Gemini mutation corresponds to a C to T transition at position 387 of the Irf8 transcript in exon 3 of 9 total exons using Genbank record NM_008320. This converts a glutamine at amino acid 118 to a stop codon removing 307 amino acids from the C-terminus of the encoded protein.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: In the spleen, there is a reduction in the frequency of mature follicular B cells and an expansion of the marginal zone B cell compartment. Complete absence of monocytes and mature macrophages in the blood, peritoneal cavity, and bone marrow. There is also expansion of granulocytes, most likely neutrophils, in the blood, spleen, and bone marrow. The mice are unable to make type I interferon (IFN) after CpG DNA challenge in vivo. Animals are susceptible to non-lethal doses of MCMV.

Heterozygous phenotype: Heterozygous animals may have an intermediate phenotype but this has not been confirmed.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Irf8m1Btlr/Irf8m1Btlr
        C57BL/6J-Irf8<m1Btlr>
  • hematopoietic system phenotype
    • decreased macrophage cell number (MGI Ref ID J:165017)
      • decreased numbers of mature macrophages
    • decreased monocyte cell number (MGI Ref ID J:165017)
    • increased neutrophil cell number (MGI Ref ID J:165017)
  • immune system phenotype
    • abnormal interferon secretion (MGI Ref ID J:165017)
      • non-secretion of type I interferon by plasmacytoid dendritic cells (pDCs) in response to in vivo stimulation by the toll-like receptor ligand, CpG
    • decreased macrophage cell number (MGI Ref ID J:165017)
      • decreased numbers of mature macrophages
    • decreased monocyte cell number (MGI Ref ID J:165017)
    • increased neutrophil cell number (MGI Ref ID J:165017)

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The Gemini strain was generated by ENU on a C57BL/6J background and was maintained on this background.

Suggested Control Mice: Wild-type littermates

Research Applications

Strain Origin

Donor: Bruce Beutler, M.D. The Scripps Research Institute.

Primary Reference:

  • Blasius AL, Arnold CN, Georgel P, Rutschmann S, Xia Y, Lin P, Ross C, Li X, Smart NG, Beutler B. Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells, Proc Natl Acad Sci U S A 2010;107(46):19973-8 (Medline PMID: 21045126)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations

Random intra-strain mating.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034294-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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