Strain Detail Sheet

Strain Name    :

C57BL/6J-Slc15a4m1Btlr/Mmjax

Stock Number :

034296-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Slc15a4m1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Slc15a4
Name: solute carrier family 15, member 4
Chromosome: 5
Alteration at locus: Chemically Induced

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mice that are homozygous for the mutation are viable and fertile. Although rare, plasmacytoid dendritic cells (pDCs) are responsible for most of the type 1 interferon (IFN) response following viral infection. pDCs from feeble mice fail to produce IFN in response to challenge from CpG-A and TLR7 or TLR9 ligands. In vitro challenge with TLR ligands abrogates secretion of IFN as well as that of other proinflammatory cytokines. In contrast, conventional dendritic cells from feeble mice produce a normal response to TLR ligands. This mutant mouse strain may be useful in studies of toll like receptor signaling and plasmacytoid dendritic cell function in the immune system.

Heterozygous phenotype: Not evaluated; heterozygote phenotype is expected to be normal.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Slc15a4m1Btlr/Slc15a4m1Btlr
        C57BL/6J-Slc15a4<m1Btlr>
  • immune system phenotype
    • abnormal interferon secretion (MGI Ref ID J:165017)
      • in vivo challenge with TLR7 or TLR9 ligands fails to produce type 1 interferon secretion in plasmacytoid dendritic cells; conventional dendritic cells respond normally to TLR ligands
    • decreased interleukin-12 secretion (MGI Ref ID J:165017)
      • in vitro challenge with TLR7 or TLR9 ligands fails to produce IL12 secretion in plasmacytoid dendritic cells
    • decreased interleukin-6 secretion (MGI Ref ID J:165017)
      • in vitro challenge with TLR7 or TLR9 ligands fails to produce IL6 secretion in plasmacytoid dendritic cells
    • decreased tumor necrosis factor secretion (MGI Ref ID J:165017)
      • in vitro challenge with TLR7 or TLR9 ligands fails to produce TNFalpha secretion in plasmacytoid dendritic cells

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results aberrant splicing due to a T to A transversion in the intron 2 donor splice site. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor: Bruce Beutler, Ph.D., The Scripps Research Institute

Primary Reference:

  • Blasius AL, Arnold CN, Georgel P, Rutschmann S, Xia Y, Lin P, Ross C, Li X, Smart NG, Beutler B, lc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells, PNAS 2010 Nov 16;107(46):19973-8 (Medline PMID: 21045126)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations

While maintaining a live colony, these mice are bred as homozygotes.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034296-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034296-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/25/2014