Allele Symbol: Slc15a4m1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Slc15a4
Name: solute carrier family 15, member 4
Alteration at locus: Chemically Induced
ES Cell Line: Not applicable
Additional mutations found on this strain:
|Allele||Source||Chr||Coord||Type||Predicted Effect||PPH Score|
|R0688:Slc15a4||UTSW||5||127608759||splice donor site||probably benign|
|R2504:Slc15a4||UTSW||5||127602018||splice acceptor site|
|R2843:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R2845:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R2846:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R2900:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R2971:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3018:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3020:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3021:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3027:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3155:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
|R3432:Slc15a4||UTSW||5||127604536||critical splice donor site||probably null|
Homozygous phenotype: Mice that are homozygous for the mutation are viable and fertile. Although rare, plasmacytoid dendritic cells (pDCs) are responsible for most of the type 1 interferon (IFN) response following viral infection. pDCs from feeble mice fail to produce IFN in response to challenge from CpG-A and TLR7 or TLR9 ligands. In vitro challenge with TLR ligands abrogates secretion of IFN as well as that of other proinflammatory cytokines. In contrast, conventional dendritic cells from feeble mice produce a normal response to TLR ligands. This mutant mouse strain may be useful in studies of toll like receptor signaling and plasmacytoid dendritic cell function in the immune system.
Heterozygous phenotype: Not evaluated; heterozygote phenotype is expected to be normal.
Strain of Origin: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results aberrant splicing due to a T to A transversion in the intron 2 donor splice site. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice: Wild-type littermates
Donor: Bruce Beutler, Ph.D., The Scripps Research Institute
While maintaining a live colony, these mice are bred as homozygotes.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.