Strain Name:
B6;129S2-E2f1tm1Njd/Mmjax
Stock Number:
034309-JAX
Citation ID:
RRID:MMRRC_034309-JAX

Gene Information

E2f1tm1Njd
Name: E2F transcription factor 1; targeted mutation 1, Nicholas J Dyson
Synonyms: E2F-1-, E2f1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
E2f1
Name: E2F transcription factor 1
Synonyms: E2F-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 13555
HGNC: HGNC:3113
Homologene: 3828
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
Homozygous: Mice that are homozygous for the targeted mutation are viable and normal in size. Although testes appear normal at 2.5 months, by 9 months homozygous males exhibit testicular atrophy and empty, degenerated seminiferous tubules, although no decrease in Leydig cells is observed. Submaxillary and parotid salivary glands develop abnormally large and swollen cell nuclei and binucleate cells. Large nuclei and binucleate cells also are observed in the exocrine portion of the pancreas. Body weights are normal until 12 to 15 months of age when many mice develop dramatic weight loss. Spontaneous tumors develop in 19% to 34% of mice between 8 and 18 months of age suggesting a function for E2f1 in tumor suppression. Tumor types include, in order of frequency, reproductive tract sarcomas, lung tumors, lymphomas and other tumor types at lower frequencies. This mutant mouse strain may be useful in studies of cancer and cell cycle research.
Heterozygous: Normal phenotype, although several heterozygotes develop rare reproductive tract sarcomas and lung adenocarcinomas.
Mammalian Phenotype Terms
Allelic Composition: E2f1tm1Njd/E2f1tm1Njd (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: E2f1tm1Njd/E2f1tm1Njd (Genetic Background: involves: 129S2/SvPas * SKH1 )

MeSH Terms
  • Animals
  • Atrophy
  • Base Sequence
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cell Division/genetics
  • Chimera
  • DNA-Binding Proteins/genetics
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Exocrine Glands/pathology
  • Exocrine Glands/physiology
  • Female
  • Gene Expression Regulation, Neoplastic/genetics
  • Genes, Tumor Suppressor/physiology
  • Lung Neoplasms/genetics
  • Lymphoma/genetics
  • Lymphoproliferative Disorders/genetics
  • Male
  • Mice
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Recombination, Genetic/physiology
  • Retinoblastoma-Binding Protein 1
  • Sarcoma, Experimental/genetics
  • Testis/pathology
  • Testis/physiology
  • Transcription Factor DP1
  • Transcription Factors/genetics
  • Cell Transformation, Neoplastic/genetics
  • DNA-Binding Proteins
  • Longevity/genetics
  • Longevity/physiology
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mutation/genetics
  • Mutation/physiology
  • Pituitary Neoplasms/genetics
  • Pituitary Neoplasms/physiopathology
  • Retinoblastoma Protein/genetics
  • Retinoblastoma Protein/physiology
  • Species Specificity
  • Thyroid Neoplasms/genetics
  • Thyroid Neoplasms/physiopathology
  • Transcription Factors/physiology
  • Apoptosis/genetics
  • DNA-Binding Proteins/deficiency
  • Gene Frequency/genetics
  • Gene Targeting/methods
  • Genes, Lethal/genetics
  • Genetic Predisposition to Disease/genetics
  • Mice, Knockout
  • Penetrance
  • Phenotype
  • Reproducibility of Results
  • Sex Factors
  • Survival Rate
  • Thymus Neoplasms/genetics
  • Transcription Factors/deficiency
  • Tumor Suppressor Protein p53/deficiency
  • Tumor Suppressor Protein p53/genetics
Strain Development
A targeting vector containing neomycin resistance gene in the antisense direction was used to replace a portion of exon 2 (cyclin A binding) and all of exon 3 (DNA binding). The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to crossed to C57BL/6J and offspring were maintained by sibling mating. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cell Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Reproduction
Donor
Lili Yamasaki, Ph.D., New York University Langone Medical Center
Primary Reference
  • Yamasaki L; Jacks T; Bronson R; Goillot E; Harlow E; Dyson NJ, Tumor induction and tissue atrophy in mice lacking E2F-1., Cell. 1996 May 17;85(4):537-48 (Medline PMID: 8653789)
  • Yamasaki L; Bronson R; Williams BO; Dyson NJ; Harlow E; Jacks T, Loss of E2F-1 reduces tumorigenesis and extends the lifespan of Rb1(+/-)mice., Nat Genet. 1998 Apr;18(4):360-4 (Medline PMID: 9537419)
  • Wloga EH; Criniti V; Yamasaki L; Bronson RT, Lymphomagenesis and female-specific lethality in p53-deficient mice occur independently of E2f1., Nat Cell Biol. 2004 Jul;6(7):565-7; author reply 567-8 (Medline PMID: 15232580)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as heterozygotes or homozygous female x heterozygous male. Homozygous males are infertile.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034309-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034309-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.