Strain Name:
C57BL/6J-Yipf6m1Btlr/Mmucd
Stock Number:
034313-UCD
Citation ID:
RRID:MMRRC_034313-UCD
Other Names:
Klein-zschocher (KlZ)
Major Collection:

Gene Information

Yipf6M1Btlr
Name: Yip1 domain family, member 6; mutation 1, Bruce Beutler
Synonyms: Klein-Zschocher, Klz
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
Yipf6
Name: Yip1 domain family, member 6
Synonyms: A430107J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 77929
Homologene: 15501
Genetic Alterations
ENU-induced T to A transversion at position 3002 of the genomic DNA sequence of Yipf6 (NC_000086.6 from NCBI Build 37.1). The dominant X-linked mutation lies in the splice acceptor site of intron 3 of the seven exon gene, and affects a thymine base five nucleotides from the next exon. cDNA sequencing revealed that the mutation results in skipping of exon 4, a frameshift, and creation of a premature stop codon at position 89 of the 236 amino acid protein.
Phenotype
Homozygous: Homozygous females and hemizygous males: KlZ mice are more susceptible to colitis induced by dextran sulfate sodium (DSS) than wild type C57BL/6J mice. When exposed to 1% DSS in the drinking water, KlZ mice lose a higher percentage of their initial weight than C57BL/6J controls.

Heterozygous: Heterozygous females: Preliminary data indicate increased susceptibility to DSS-induced colitis relative to C57BL/6J mice. The phenotype of heterozygous females may be more variable due to X-inactivation.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
Original mutant was a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice

Wild-type littermates

  • Cell Biology
  • Immunology and Inflammation
Donor
Bruce Beutler, M.D., while at The Scripps Research Institute
Primary Reference

Arnold CN, Eidenschenk C,  Huber C, Kalina S, Krebs P, Lin P, Moresco EY, Murray A,
Nemazee D,  Pirie E, Purrington T, Shi H, Smart NG, Siggs OM, Tomisato W, Wang Y, Won S, Xia Y,
Beutler B.

Authors, Science Writers, Beutler B. Record for Beutler allele name. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Authors, Science Writers, Beutler B. Record for Beutler allele name. MUTAGENETIX, B. Beutler and colleagues, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Random intra-strain mating.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034313-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.