Strain Name:
B6;129S2-Tfdp1tm1Lili/Mmjax
Stock Number:
034318-JAX
Citation ID:
RRID:MMRRC_034318-JAX

Gene Information

Tfdp1tm1Lili
Name: transcription factor Dp 1; targeted mutation 1, Lili Yamasaki
Synonyms: Dp1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Targeted Mutation
Tfdp1
Name: transcription factor Dp 1
Synonyms: Dp1, Drtf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Targeted Mutation
NCBI: 21781
Homologene: 38276
Genotype Determination
ES Cell Line
D3 Derived From 129S2/SvPas
Phenotype
Homozygous: Embryos die before E12.5 due to the failure of extra-embryonic development. Although expected Mendelian numbers of embryos are recovered at E9.5, embryos are developmentally delayed and small in size. At E8.5 extra-embryonic trophoectoderm-derived tissues, in particular, the ectoplacental cone and trophoblast giant cells are under-represented and exhibit DNA replication defects. This mutant mouse strain may be useful in studies of early embryonic development and infertility related to placental failure.

Heterozygous: Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Tfdp1tm1Lili/Tfdp1tm1Lili (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Tfdp1tm1Lili/Tfdp1+ (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

MeSH Terms
  • Animals
  • Cell Cycle Proteins/biosynthesis
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/metabolism
  • DNA-Binding Proteins/biosynthesis
  • DNA-Binding Proteins/genetics
  • E2F Transcription Factors
  • E2F1 Transcription Factor
  • Fetal Death/genetics
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Genes, Lethal
  • Mice
  • Mice, Knockout
  • Transcription Factor DP1
  • Transcription Factors/biosynthesis
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Trophoblasts/metabolism
  • Tumor Suppressor Protein p53/metabolism
  • Cell Cycle/physiology
  • Chimera/anatomy & histology
  • Chimera/physiology
  • Embryo, Mammalian/physiology
  • Embryonic and Fetal Development
  • Female
  • Gene Expression Profiling
  • Genetic Markers
  • Gestational Age
  • Male
  • Mice, Inbred C57BL
  • Oligonucleotide Array Sequence Analysis
  • Pluripotent Stem Cells/physiology
  • Pregnancy
  • Proteins/genetics
  • Proteins/metabolism
  • RNA, Untranslated
Strain Development
A targeting vector containing neomycin resistance gene in the antisense direction was used to replace exons 4 through 9, a region encoding the DNA binding and heterodimerization domains. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J and offspring were maintained by sibling mating. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Reproduction
Donor
Lili Yamasaki, Ph.D., New York University Langone Medical Center
Primary Reference
  • Kohn MJ; Bronson RT; Harlow E; Dyson NJ; Yamasaki L, Dp1 is required for extra-embryonic development., Development. 2003 Apr;130(7):1295-305 (Medline PMID: 12588846)
  • Kohn MJ; Leung SW; Criniti V; Agromayor M; Yamasaki L, Dp1 is largely dispensable for embryonic development., Mol Cell Biol. 2004 Aug;24(16):7197-205 (Medline PMID: 15282318)

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are not viable.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034318-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
034318-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.