Strain Detail Sheet

Strain Name    :

B6.129S7-Mdm2tm1Ypz/Mmnc

Stock Number :

034319-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Mdm2tm1Ypz
Name: targeted mutation 1, Yanping Zhang
Alteration at locus: Knock-In
Target: Mdm2
Name: transformed mouse 3T3 cell double minute 2
Chromosome: 10
Alteration at locus: Knock-In

Genetic Alterations:
TGT to GCC substitution at codon 462 resulting in a Cys>Ala substitution at the zinc- coordinating residue C462 in mouse Mdm2.

Genotype Determination:

ES Cell Line: AB2.2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Embryonic lethal at E7.5.

Heterozygous phenotype: Normal and fertile.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mdm2tm1Ypz/Mdm2tm1Ypz
        involves: 129S7/SvEvBrd * C57BL/6 * FVB/N
  • mortality/aging
    • complete embryonic lethality before somite formation (MGI Ref ID J:126004)
      • mice die prior to E7.5
  • embryogenesis phenotype
    • abnormal gastrulation (MGI Ref ID J:126004)
      • embryos fail to undergo proper gastrulation
    • decreased embryo size (MGI Ref ID J:126004)
      • at E7.5
    • embryonic growth retardation (MGI Ref ID J:126004)
      • at E7.5 embryos exhibit a size and structure similar to E6.5 embryos
  • growth/size phenotype
    • decreased embryo size (MGI Ref ID J:126004)
      • at E7.5
    • embryonic growth retardation (MGI Ref ID J:126004)
      • at E7.5 embryos exhibit a size and structure similar to E6.5 embryos

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: Backcrossed with C57BL/6 breeders.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129S7-Mdm2tm1Ypz MMRRC:034319-UNC C57BL/6J 129S7/AB2.2 2412 0 170 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Models for Human Disease

Strain Origin

Donor: Yanping Zhang, Ph.D., University of North Carolina at Chapel Hill

Primary Reference:

Itahana K et al., "Targeted Inactivation of Mdm2 RING finger E3 ubiquitin ligase activity in the mouse reveals mechanistic insights into p53 regulation." Cancer Cell 2007 Oct;12(4):355-66 (Medline PMID: 17936560)

Colony and Husbandry Information

Special Considerations

Backcross to C57BL/6.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034319-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034319-UNC-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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