Strain Detail Sheet

Strain Name    :

B6.129P2-Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk/Mmmh

Stock Number :

034320-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Gt(ROSA)26Sortm1(Eif1a-tTA,tetO-mCherry/HTR4*D100A)Conk
Name:
Alteration at locus: Knock-In
Target: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Alteration at locus: Knock-In

Genetic Alterations:
E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. ES cells remain on the 129P2/OlaHsd background.

Genotype Determination:

ES Cell Line: E14 derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Hemizygous mice have increased mineralization in calvaria.

Strain of Origin: 129P2/OlaHsd

Strain genetic background: B6.129P2

Strain Development: E14Tg2A.4 mouse embryonic stem cells were electroporated with a Rosa26 vector carrying the combined EF1a-tTA and TetO-mCh-Rs1 transgenes in a single construct. Mice were generated by blastocyst injection, and chimeras were backcrossed to C57BL/6.

Suggested Control Mice: Wild-type littermates or C57BL/6

Research Applications

  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Metabolism

Strain Origin

Donor: Bruce Conklin, M.D., Gladstone Institute of Cardiovascular Disease

Primary Reference:

Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR.  Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.  Stem Cell Res Ther. 2011 Mar 4;2(2):11.  (Medline PMID: 21375737).

Colony and Husbandry Information

Special Considerations

Doxycycline diet = BioServ DoxDiet 200 mg/kg.  No embryonic lethality was identifed when mated off of doxycycline (with transgenes induced). However, the donor recommends maintining the mouse line on doxycycline (or maintaining the ES cell line in doxy-containing media) to prevent any unexpected effects from Rs1 expression.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • C57BL/6 female x Hemizygous male or reciprocol mating.
  • Note: Donor reports that attemts to backross to 129P2/OlaHsd produced poor litter sizes.

Generation: N6

Overall Breeding Performance: Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMale
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: 7

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034320-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034320-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014