Strain Detail Sheet

Strain Name    :

B6;129S4-Myh9tm3(GFP/MYH9/MYH10)Rsad/Mmnc

Stock Number :

034322-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Myh9tm3(GFP/MYH9/MYH10)Rsad
Name: targeted mutation 3, Robert S Adelstein
Alteration at locus: Knock-In
Target: Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Chromosome: 15
Alteration at locus: Knock-In

Genetic Alterations:
cDNA encoding GFP- nonmuscle myosin IIA head fused to IIB rod was inserted into exon2 of the nonmuscle myosin IIA heavy chain (Myh9) gene locus, placing a chimeric nonmuscle myosin IIA/B under control of the IIA promoter. Neo cassette was placed into intron.

Genotype Determination:

ES Cell Line: v6.5

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Homozygous mice die at E10.5-11.5 with defects in placenta formation and angiogenesis.

Heterozygous phenotype: Heterozygous mice are normal for all tested phenotypes and are fertile.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Myh9tm3(GFP/MYH9/MYH10)Rsad/Myh9tm3(GFP/MYH9/MYH10)Rsad
        involves: 129S4/SvJae * C57BL/6
  • mortality/aging
    • complete embryonic lethality during organogenesis (MGI Ref ID J:163702)
      • die between E11.5 and E12.5
  • embryogenesis phenotype
    • embryogenesis phenotype (MGI Ref ID J:163702)
      • unlike null mice, knock in embryos undergo gastrulation
      • abnormal placenta labyrinth morphology (MGI Ref ID J:163702)
        • thinner compared to controls
        • abnormal placental labyrinth vasculature morphology (MGI Ref ID J:163702)
          • invasion of embryonic blood vessels is impaired
          • decrease in the internal space of fetal and maternal vessels
      • abnormal vitelline vasculature morphology (MGI Ref ID J:163702)
        • fewer visible vessels at E9.5 and E10.5
      • pale yolk sac (MGI Ref ID J:163702)
  • cellular phenotype
    • abnormal cell adhesion (MGI Ref ID J:163702)
      • fewer and smaller focal adhesions are detected in MEFs and the stress fibers in the focal adhesions are in disarray
    • abnormal cell content or morphology (MGI Ref ID J:163702)
      • i n MEFs actin filaments are thin, disorganized and often fail to align with the direction of migration
    • abnormal cell migration (MGI Ref ID J:163702)
      • in a wound healing assay MEFs close the wound more rapidly compared to wild-type controls
      • a transwell assay indicates that directional cell migration is impaired in MEFs
  • cardiovascular system phenotype
    • abnormal vitelline vasculature morphology (MGI Ref ID J:163702)
      • fewer visible vessels at E9.5 and E10.5

Strain of Origin: 129

Strain genetic background: B6;129

Strain Development: V6.5 embryonic stem cells were injected into C57BL/6 blastocysts and chimeras were born. Chimeras were mated to C57BL/6 wild type mice to generate heterozygotes. Strain is maintained as heterozygotes.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129S4-Myh9tm3(GFP/MYH9/MYH10)Rsad/Mmnc MMRRC:034322-UNC C57BL/6J 129S4/AK7 2228 79 274 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Reproduction
  • Research Tools

Strain Origin

Donor: Robert S. Adelstein, M.D., NIH/NHLBI

Primary Reference:

Wang A; Ma X; Conti MA; Liu C; Kawamoto S; Adelstein RS, Nonmuscle myosin II isoform and domain specificity during early mouse development., Proc Natl Acad Sci U S A 2010 Aug 17;107(33):14645-50 (Medline PMID: 20679233)

Colony and Husbandry Information

Special Considerations

Strain is maintained as heterozygotes.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034322-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034322-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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