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Strain Detail Sheet

Strain Name:
CAST.129S1(B6)-Kif18agcd2/JcsMmjax
Stock Number:
034325-JAX
Citation ID:
RRID:MMRRC_034325-JAX

Strain Information

Gene Details

Kif18agcd2
Name: kinesin family member 18A; germ cell depletion, John Schimenti
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Kif18a
Name: kinesin family member 18A
Synonyms: N-8 kinesin, B130001M12Rik, gcd2
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228421
Homologene: 41820

Additional Resources
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GeneCards
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Genetic Alterations
The germ cell depletion 2 mutation was induced in 129S1/Sv-Oca2+ Tyr+ Kitl+-derived CJ7 ES cells via a 16 hour exposure to ethyl methansulfonate.  Treated ES cells were injected into C57BL/6J blastocysts and the chimeras were bred with C57BL/6J males.  The mutation results in a G to A transversion in exon 7 of the Kif18a gene and alters the corresponding amino acid from arginine to lysine at codon 308 (R308K).
Genotype Determination

Strain Description

Phenotype
Homozygotes: Mice that are homozygous for this mutation exhibit a strain dependent sterility phenotype due to germ cell depletion. On the CAST/Ei background, homozygotes are 90% sterile.  Testes have few or no developing germ cells and ovaries are small with few or no follicles.  The reduction in the number of germ cells is first observed in the embryonic proliferative/post-proliferative period (11.5 dpc) following germ cell migration to the embryonic gonads.  On a C57BL/6J background, 37% of mice are infertile and on a mixed CAST/Ei and C57BL/6J background, an intermediate phenotype (64%) is observed.  This mutant mouse strain may be useful in studies of gametogenesis.
Heterozygote:  Not evaluated. The heterozygote phenotype is expected to be similar to the wild-type phenotype.
Mammalian Phenotype Terms
Allelic Composition: gcd2/gcd2 (Genetic Background: B6.129S1-gcd2/JcsJ )
Allelic Composition: gcd2/gcd2 (Genetic Background: CAST.129S1(B6)-gcd2/JcsJ )
Allelic Composition: gcd2/gcd2 (Genetic Background: involves: 129S1/Sv * C57BL/6 )
MeSH Terms
  • Animals
  • Embryonic Stem Cells/pathology
  • Female
  • Infertility, Female/genetics
  • Infertility, Male/genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mutation
  • Ovum/pathology
  • Spermatozoa/pathology
  • Chromosome Mapping
  • Ethylnitrosourea/pharmacology
  • Gametogenesis/genetics
  • Genotype
  • Infertility/genetics
  • Meiosis/genetics
  • Mutagenesis/drug effects
  • Mutagenicity Tests
  • Mutagens/pharmacology
  • Mutation/genetics
  • Oocytes/physiology
  • Phenotype
  • Pregnancy
  • Reproduction/genetics
Strain Development
CJ7 (129S1/SvImJ) were mutagenized with ethylmethanesulfonate (EMS). The ES cells were injected into C57BL/6J host embryos. The resulting chimeras were mated to C57BL/6J females. The resulting G1s were outcrossed to C57BL/6J. The G2 females were then backcrossed to their G1 fathers and the G3 animals were screened for fertility. The gcd2 mice are derived from one such family, which yielded 1/4 sterile G3 offspring. Confirmed carrier G2 and G3 males were mated with Cast/EiJ females for recombination mapping. F2 animals from this cross were backcrossed to Cast/EiJ to generate a congenic line. This breeding scheme was followed for 8 generations and the sex of the donor parent was alternated at each generation.
Suggested Control Mice
Wild-type littermates or Cast/EiJ

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cancer
  • Developmental Biology
  • Reproduction

Strain Origin

Donor
Laura Reinholdt, The Jackson Laboratory.
Primary Reference
  • Reinholdt LG, Munroe RJ, Kamdar S, Schimenti JC. The mouse gcd2 mutation causes primordial germ cell depletion. Mech Dev 2006 Jul;123(7):559-69. (Medline PMID:16822657)
  • Ward JO, Reinholdt LG, Hartford SA, Wilson LA, Munroe RJ, Schimenti KJ, Libby BJ, O'Brien M, Pendola JK, Eppig J, Schimenti JC. Toward the genetics of mammalian reproduction: induction and mapping of gametogenesis mutants in mice., Biol Reprod 2003 Nov;69(5):1615-25. (Medline PMID:12855593).

Colony and Husbandry Information

Special Considerations

Please note: Because in vitro fertilization is problematic with CAST oocytes, mice recovered from cryopreservation will be generated using C57BL/6 oocytes. Recovery of the 90% sterility phenotype will require additional crosses to CAST.

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Agouti

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Heterozygous female x Heterozygous male

Generation
N9F4
Overall Breeding Performance
Unknown

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 months 10 months
Average litter size
5
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
034325-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
034325-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / $2,123.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.