Strain Detail Sheet

Strain Name    :

C57BL/6J-Atp11cm2Btlr/Mmjax

Stock Number :

034371-JAX

Other Names   :

spelling

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Atp11cm2Btlr
Name: mutation 2, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Atp11c
Name: ATPase, class VI, type 11C
Chromosome: X
Alteration at locus: Chemically Induced

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. However, more than half of all homozygous females suffer dystocia after pregnancy. Mice exhibit reduced numbers of CD19+ B cells. The reduction in B cells is first observed at the pre-pro B to pro-B transition in the bone marrow. Marginal zone B cells persist in normal numbers. The donating investigator reports that mice develop hyperbilirubinemia with elevated levels of cholic acid in the serum. This mutant mouse strain may be useful in studies of B cell lymphopoiesis and cholestasis.

Heterozygous phenotype: normal

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a T to A transversion at position 1214 in exon 12 of 30 exons. The mutation alters the corresponding amino acid from isoleucine to lysine at codon 355. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Suggested Control Mice: Wild-type littermates or C57BL/6J mice

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor: Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference:

  • Siggs OM, Schnabl B, Webb B, Beutler B. X-linked cholestasis in mouse due to mutations of the P4-ATPase ATP11C.  Proc Natl Acad Sci U S A. 2011 May 10;108(19):7890-5. Epub 2011 Apr 25. (Medline PMID:21518881).
  • Arnold CN, Pirie E, Smart NG, Beutler B. Record for spelling. MUTAGENETIX™, B. Beutler and colleagues, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations

The donating investigator maintains these mice as heterozygotes. Homozygous females are fertile, but have a high incidence of dystocia.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System:

Breeding Scheme(s):

Generation:

Overall Breeding Performance:

Reproductive Statistics

Viability and Fertility: Unknown

Average litter size:

Recommended wean age:

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034371-JAX-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
034371-JAX-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014