Strain Name:
B6NCr.129P2-Akap13Gt(AG0213)Wtsi/Mmucd
Stock Number:
034375-UCD
Citation ID:
RRID:MMRRC_034375-UCD
Other Names:
AKAP13-deltaBrx

Strain Information

Akap13Gt(AG0213)Wtsi
Name: A kinase anchor protein 13; gene trap AG0213, Wellcome Trust Sanger Institute
Synonyms: deltaBrx
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Gene Trap
Akap13
Name: A kinase anchor protein 13
Synonyms: AKAP-Lbc, PROTO-LBC, PROTO-LB, Ht31, 5730522G15Rik, 1700026G02Rik, 5830460E08Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Gene Trap
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Genetic Alterations
This strain harbors a gene-trap mutation from the Sanger Gene Trap Resource (AG0213) that inserts the pGT0lxr vector between the alternative promoter exon for the Akap13 transcript Brx and exon 9. The trapping event causes a fusion of the upstream AKAP13 protein with the betaGeo cassette.
Genotype Determination

ES Cell Line
E14TG2a.4
Phenotype
The Donating Investigator reports that other AKAP13-null allele mice die during development; however, homozygotes for this allele survive normally and are fertile. AKAP13 is broadly expressed in mouse heart, vasculature, lung, and brain, and it has been shown to regulate cardiac hypertrophy and is linked to defects in eye development, Alzheimer's Disease, and cancer. This mutant may help delineate the transcript specific roles of AKAP13 in mediating stress responses several systems - heart, brain, vasculature, lung, and kidney.This mutation will help delineate the transcript specific roles of AKAP13 in mediating stress responses.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
Correctly targeted ES cells (E14TG2a.4) were injected into C57BL/6J host blastocysts to generate chimeras. The chimeras were then crossed with C57BL/6NCr mice to determine germline transmission. The strain was maintained by continuous backcrossing to C57BL/6NCr for 5 generations.
Suggested Control Mice
Wild-type littermates or C57BL/6NCr
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Endocrined Deficiency
  • Immunology and Inflammation
  • Models of Human Disease
  • Reproduction
Donor
Bruce Conklin, M.D., Ph.D., Gladstone Institute of Cardiovascular Disease.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6NCr female x Heterozygous male or reciprocal
Generation
N5
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
7
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034375-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034375-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
034375-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.