Strain Detail Sheet

Strain Name    :

B6.129P2-Boctm1Aok/Mmucd

Stock Number :

034378-UCD

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Boctm1Aok
Name: targeted mutation 1, Ami Okada
Alteration at locus: Targeted Mutation
Gene Symbol: Boc
Name: biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein
Chromosome: 16
Alteration at locus: Targeted Mutation

Genetic Alterations:
A cassette encoding a splice acceptor followed by beta-geo and ires-hPLAP was introduced into the intron in between the two exons preceding the transmembrane domain by homologous recombination.

Genotype Determination:

ES Cell Line: E14

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Problems in axon guidance of spinal cord commisural neurons during development, problems in retinal gangion cell axon projection during development.
Heterozygous phenotype: None.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Boctm1Aok/Boctm1Aok
        involves: 129P2/OlaHsd * CD-1
  • nervous system phenotype
    • nervous system phenotype (MGI Ref ID J:115577)
      • at E11.5 neural tube patterning appears normal
      • abnormal innervation (MGI Ref ID J:115577)
        • at E11.5 TAG+ commissural neuron projects are highly dispersed and invade the ventral spinal cord with ectopic projections extending over the motor columns

Strain of Origin: 129P2/OlaHsd

Strain genetic background: B6.129P2

Strain Development: Chimaeric mice were generated from the targeted ES cells, and offspring transmitting the mutant allele were crossed to C57BL/6J mice, and subsequent heterozygous offspring were crossed to wild-type C57BL/6J mice for 6 generations.

Suggested Control Mice:

  • Wild-type littermates or C57BL/6J

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Neurobiology

Strain Origin

Donor: Ami Okada, PhD, Stanford University

Primary Reference:

  • Okada A, Charron F, Morin S, Shin DS, Wong K, Fabre PJ, Tessier-Lavigne M, McConnell SK. Boc is a receptor for sonic hedgehog in the guidance of commissural axons. Nature. 2006 Nov 16;444(7117):369-73 (Medline PMID: 17086203).
  • Fabre PJ, Shimogori T, Charron F. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc. J Neurosci. 2010 Jan 6;30(1):266-75. (Medline PMID: 20053908).
  • Harwell CC, Parker PR, Gee SM, Okada A, McConnell SK, Kreitzer AC, Kriegstein AR. Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation. Neuron. 2012 Mar 22;73(6):1116-26. Epub 2012 Mar 21. (Medline PMID: 22445340).

Colony and Husbandry Information

Special Considerations

Mice used to propagate the strain were always bred to C57BL/6 mice, while mice used for experiments were derived from brother-sister matings.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • C57BL/6J female x Heterozygous male or recipricol mating

Generation: N>6

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 6 months 6 months

Average litter size: 5

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
034378-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
034378-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
034378-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/17/2014