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Strain Name:
B6.129P2-Boctm1Aok/Mmucd
Stock Number:
034378-UCD
Citation ID:
RRID:MMRRC_034378-UCD
Other Names:
Boc

Strain Information

Boctm1Aok
Name: BOC cell adhesion associated, oncogene regulated; targeted mutation 1, Ami Okada
Synonyms: Boc-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Targeted Mutation
Boc
Name: BOC cell adhesion associated, oncogene regulated
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Targeted Mutation
NCBI: 117606
Homologene: 32819
Genetic Alterations
A cassette encoding a splice acceptor followed by beta-geo and ires-hPLAP was introduced into the intron in between the two exons preceding the transmembrane domain by homologous recombination.
Genotype Determination

ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype
Homozygous: Problems in axon guidance of spinal cord commisural neurons during development, problems in retinal gangion cell axon projection during development.
Heterozygous: No observed phenotype.
Mammalian Phenotype Terms
Allelic Composition: Boctm1Aok/Boctm1Aok (Genetic Background: involves: 129P2/OlaHsd * CD-1 )

MeSH Terms
  • Animals
  • Axons/physiology
  • COS Cells
  • Cell Adhesion Molecules/deficiency
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism
  • Cercopithecus aethiops
  • Hedgehog Proteins/metabolism
  • Immunoglobulin G/genetics
  • Immunoglobulin G/metabolism
  • Mice
  • Protein Binding
  • RNA Interference
  • Receptors, Cell Surface/deficiency
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/metabolism
  • Cells, Cultured
  • Female
  • Hedgehog Proteins/physiology
  • Humans
  • Immunoglobulin G/physiology
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Optic Chiasm/embryology
  • Optic Chiasm/physiology
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cell Surface/physiology
  • Retinal Ganglion Cells/physiology
  • Visual Pathways/embryology
  • Visual Pathways/physiology
  • Age Factors
  • Animals, Newborn
  • Cerebral Cortex/cytology
  • Cerebral Cortex/growth & development
  • Channelrhodopsins
  • Corpus Callosum/cytology
  • Corpus Callosum/growth & development
  • DNA-Binding Proteins/metabolism
  • Dendritic Spines/metabolism
  • Dendritic Spines/physiology
  • Electric Stimulation
  • Electroporation/methods
  • Fluorobenzenes/metabolism
  • Functional Laterality/genetics
  • Furans/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology
  • Hedgehog Proteins/genetics
  • In Vitro Techniques
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Matrix Attachment Region Binding Proteins/metabolism
  • Membrane Potentials/genetics
  • Mice, Transgenic
  • Mutation/genetics
  • Nerve Net/cytology
  • Nerve Net/metabolism
  • Neurons/metabolism
  • Neurons/ultrastructure
  • Nuclear Proteins/metabolism
  • Patch-Clamp Techniques
  • Phosphopyruvate Hydratase/metabolism
  • Pyramidal Tracts/physiology
  • RNA, Small Interfering/genetics
  • RNA, Small Interfering/metabolism
  • Repressor Proteins/metabolism
  • Silver Staining/methods
  • Stilbamidines/metabolism
  • Synapses/metabolism
  • Synapses/ultrastructure
  • Synaptophysin/genetics
  • Synaptophysin/metabolism
  • Transcription Factors/metabolism
  • Tumor Suppressor Proteins/metabolism
  • gamma-Aminobutyric Acid/metabolism
Strain Development
Chimaeric mice were generated from the targeted ES cells, and offspring transmitting the mutant allele were crossed to C57BL/6J mice, and subsequent heterozygous offspring were crossed to wild-type C57BL/6J mice for 6 generations.
Suggested Control Mice

Wild-type littermates or C57BL/6J

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Neurobiology
Donor
Ami Okada, PhD, Stanford University
Primary Reference
  • Okada A, Charron F, Morin S, Shin DS, Wong K, Fabre PJ, Tessier-Lavigne M, McConnell SK. Boc is a receptor for sonic hedgehog in the guidance of commissural axons. Nature. 2006 Nov 16;444(7117):369-73 (Medline PMID: 17086203).
  • Fabre PJ, Shimogori T, Charron F. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc. J Neurosci. 2010 Jan 6;30(1):266-75. (Medline PMID: 20053908).
  • Harwell CC, Parker PR, Gee SM, Okada A, McConnell SK, Kreitzer AC, Kriegstein AR. Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation. Neuron. 2012 Mar 22;73(6):1116-26. Epub 2012 Mar 21. (Medline PMID: 22445340).

Colony and Husbandry Information

Mice used to propagate the strain were always bred to C57BL/6 mice, while mice used for experiments were derived from brother-sister matings.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6J female x Heterozygous male or recipricol mating
Generation
N>6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 6 months
Average litter size
5
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034378-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,568.61
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034378-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $882.81
Non-Profit / For-Profit
Aliquot Approximate quantity3
034378-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.