Strain Name:
B6.129P2-Cdontm1Aok/Mmucd
Stock Number:
034379-UCD
Citation ID:
RRID:MMRRC_034379-UCD
Other Names:
Cdon

Strain Information

Cdontm1Aok
Name: cell adhesion molecule-related/down-regulated by oncogenes; targeted mutation 1, Ami Okada
Synonyms: Cdon-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Targeted Mutation
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CDO, CAM-related/down-regulated by oncogenes
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Targeted Mutation
NCBI: 57810
Homologene: 22996
Genetic Alterations
A cassette encoding beta-geo and ires-hPLAP preceded by a splice acceptor site and followed by a transcription stop/poly-adenylation signal was inserted into an intron that is between the two exons preceding the exon encoding the transmembrane domain (between exons 13 and 14). Thus both beta- galactosidase, which labels the cell body, and human placental Alkaline phosphatase, which labels axons and processes, could be used as markers of the cells.
Genotype Determination

ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype
Homozygous: Mild craniofacial defects sometimes associated with mislaligned jaws resulting in elongated incisors, and occasionally a single central incisor ('micro' form of holoprosencephaly); skin and muscle tissue feels thinner. A fraction of the homozygous offspring (~25%) exhibit hydrocephalus; often the mutant is runty to about 3 weeks, occasionally longer. Some male homozygotes exhibited a protrusion of the penis, though recent homozygotes have not exhibited this phenotype.
Heterozygous: No observed phenotype.
Mammalian Phenotype Terms
Allelic Composition: Cdontm1Aok/Cdontm1Aok (Genetic Background: involves: 129P2/OlaHsd * CD-1 )

MeSH Terms
  • Animals
  • Axons/physiology
  • COS Cells
  • Cell Adhesion Molecules/deficiency
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism
  • Cercopithecus aethiops
  • Hedgehog Proteins/metabolism
  • Immunoglobulin G/genetics
  • Immunoglobulin G/metabolism
  • Mice
  • Protein Binding
  • RNA Interference
  • Receptors, Cell Surface/deficiency
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • Spinal Cord/cytology
  • Spinal Cord/metabolism
Strain Development
Chimaeric mice were generated from the targeted ES cells, and offspring transmitting the mutant allele were crossed to C57BL/6 mice, and subsequent heterozygous offspring were crossed to wild-type C57BL/6 mice for 6 generations. Prior to submission to MMRRC, the colony was established with occasional sib breeding, but intra-strain mating to offspring derived from different breedings were preferentially performed. To maintain the line, heterozygotes were crossed to the wild-type strain, C57BL/6 obtained from the vendor; to avoid in-breeding as much as possible at least two sources of such heterozygote to wild-type pairs were maintained.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Ami Okada, Ph.D., Stanford University
Primary Reference
Okada A, Charron F, Morin S, Shin DS, Wong K, Fabre PJ, Tessier-Lavigne M, McConnell SK. Boc is a receptor for sonic hedgehog in the guidance of commissural axons. Nature. 2006 Nov 16;444(7117):369-73. (Medline PMID: 17086203).

Colony and Husbandry Information

Some homozygote pups have craniofacial defects resulting in overgrowth or lack of one or both incisors; trimming of the teeth may be necessary, as well as including water mashed food in the cage for their consumption.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6J female x Heterozygous male or recipricol mating
Generation
N>6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No Reduced Donor reports that about 30% of male homozygotes could be fertile, but most did not mate.
Heterozygotes are fertile: Unknown Unknown
Age Reproductive Decline: 6 months 6 months
Average litter size
5
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034379-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
034379-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
034379-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.