Strain Name:
B6.129S-Neurog3tm1(EGFP)Khk/Mmucd
Stock Number:
000344-UCD
Citation ID:
RRID:MMRRC_000344-UCD
Other Names:
ngn3-EGFP

Gene Information

Allele Symbol: Neurog3tm1(EGFP)Khk (Mus musculus (mouse))
Name: neurogenin 3; targeted mutation 1, Klaus H Kaestner
Synonyms: ngn3-, Ngn3-eGFP, Neurog3
Chromosome: 10
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:2651394
Gene Symbol: Neurog3 (Mus musculus (mouse))
Name: neurogenin 3
Synonyms: bHLHa7, Math4B, ngn3, Atoh5
Chromosome: 10
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:893591
Genetic Alterations
EGFP replaces entire coding region of Neurog3
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
EGFP expression in developing endocrine cells of pancreas and in glandular regions of the stomach.
Mammalian Phenotype Terms
Allelic Composition: Neurog3tm1(EGFP)Khk/Neurog3tm1(EGFP)Khk (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

MeSH Terms
  • Alleles
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • DNA-Binding Proteins/metabolism
  • Digestive System/metabolism
  • Enterochromaffin Cells/metabolism
  • Enteroendocrine Cells/metabolism
  • Epithelial Cells/metabolism
  • Gastric Mucosa/pathology
  • Gastrins/biosynthesis
  • Gene Expression Regulation, Developmental
  • Glucagon/biosynthesis
  • Glucagon/metabolism
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Luminescent Proteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron
  • Microscopy, Fluorescence
  • Models, Genetic
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Oligonucleotide Array Sequence Analysis
  • RNA/metabolism
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Somatostatin/biosynthesis
  • Stomach/cytology
  • Stomach/metabolism
  • Transcription Factors/metabolism
Suggested Control Mice
Wild-type littermates
  • Diabetes/Obesity
  • Endocrine Deficiency
Donor
Klaus Kaestner Ph.D., University of Pennsylvania
Primary Reference
Lee CS, Perreault N, Brestelli JE, Kaestner KH. Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity. Genes Dev. 2002 Jun 15;16(12):1488-97. (Medline PMID: 12080087)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
MMRRC Breeding System
Intrastrain
Breeding Scheme(s)
  • Heterozygous female x Wild type male
Generation
Unknown
Overall Breeding Performance
Normal
Average litter size
6 - 8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000344-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
000344-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
000344-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.