Strain Name:
B6;129-Vegfatm1Pec/Mmjax
Stock Number:
034407-JAX
Citation ID:
RRID:MMRRC_034407-JAX

Gene Information

Vegfatm1Pec
Name: vascular endothelial growth factor A; targeted mutation 1, Peter Carmeliet
Synonyms: VEGF120
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knock-In
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VPF, VEGF-A, VEGF120, Vegf, VEGF188, VEGF164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knock-In
NCBI: 22339
Homologene: 2534
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
These VEGF120mice express a VEGF isoform that lacks exons 6 and 7 (including the extracellular matrix binding structures) resulting in a protein that is highly soluble. The majority of homozygous pups die shortly after birth due to cardiorespiratory distress; survivors do not live beyond two weeks of age due to impaired angiogenesis resulting in cardiac failure. Homozygotes exhibit severe retinal vascular defects including a primitive vascular labyrinth at post natal day 5 (P5), delayed periendothelial cell recruitment and reduced numbers of pericytes. In mice that survive to P9, the capillary bed covers two thirds of the retina. Arteriole and venule development is reduced and capillary number is increased. Capillaries are dilated and fragile resulting in hemorrhages. Hyaloid arteries appear dilated and tortuous with no connections to the retinal vasculature. Hyaloid regression is not observed by P9. Numbers of renal and cornary arteries are reduced. This mutant mouse strain may be useful in studies of retinal vascular outgrowth and patterning.

The Donating Investigator reports that very few mice survive beyond post natal day 1.

Heterozygous: Heterozygous mice are viable, fertile and normal in size.

Mammalian Phenotype Terms
Allelic Composition: Vegfatm1Pec/Vegfatm1Pec (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • Arterioles/abnormalities
  • Arterioles/growth & development
  • Base Sequence
  • Body Patterning/genetics
  • Body Patterning/physiology
  • DNA/genetics
  • Endothelial Growth Factors/genetics
  • Endothelial Growth Factors/physiology
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Lymphokines/genetics
  • Lymphokines/physiology
  • Male
  • Mice
  • Mice, Transgenic
  • Pregnancy
  • Protein Isoforms/genetics
  • Protein Isoforms/physiology
  • Receptor Protein-Tyrosine Kinases/genetics
  • Receptors, Growth Factor/genetics
  • Receptors, Vascular Endothelial Growth Factor
  • Retinal Vessels/abnormalities
  • Retinal Vessels/growth & development
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Venules/abnormalities
  • Venules/growth & development
  • Alternative Splicing
  • Antigens, Differentiation
  • Disease Models, Animal
  • Endothelial Growth Factors/deficiency
  • Endothelium, Vascular/chemistry
  • Heart Function Tests
  • Lymphokines/deficiency
  • Mice, Mutant Strains
  • Muscle, Skeletal/blood supply
  • Myocardial Ischemia/pathology
  • Myocardium/pathology
  • Neovascularization, Physiologic/genetics
  • Protein Isoforms/deficiency
Strain Development
A targeting vector containing a loxP-flanked neomycin cassette was used to replace exons 6 and 7. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to C57BL/6 and then maintained by sibling mating. The VEGF 120 isoform lacks exons 6 and 7, which includes the extracellular matrix binding structures and is highly soluble. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
Donor
Patricia D'Amore, Ph.D., Schepens Eye Research Institute.
Primary Reference

Stalmans I; Ng YS; Rohan R; Fruttiger M; Bouche A; Yuce A; Fujisawa H; Hermans B; Shani M; Jansen S; Hicklin D; Anderson DJ; Gardiner T; Hammes HP; Moons L; Dewerchin M; Collen D; Carmeliet P; D'Amore PA, Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms., J Clin Invest. 2002 Feb;109(3):327-36 (Medline PMID: 11827992)

Carmeliet P; Ng YS; Nuyens D; Theilmeier G; Brusselmans K; Cornelissen I; Ehler E; Kakkar VV; Stalmans I; Mattot V; Perriard JC; Dewerchin M; Flameng W; Nagy A; Lupu F; Moons L; Collen D; D'Amore PA; Shima DT, Impaired myocardial angiogenesis and ischemic cardiomyopathy in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188 [see comments], Nat Med. 1999 May;5(5):495-502 (Medline PMID: 10229225)

Colony and Husbandry Information

When maintaining a live colony, these mice may be bred as heterozygotes. Most homozygotes die shortly after birth due to cardiorespiratory distress, survivors die within two weeks of age due to cardiac failure.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
034407-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
034407-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.