Allele Symbol: Mapttm1Hnd
Name: targeted mutation 1, Hana N Dawson
Alteration at locus: Knockout
Gene Symbol: Mapt
Name: microtubule-associated protein tau
Alteration at locus: Knockout
Name: transgene insertion 4, Gerard Schellenberg
Alteration at locus: Transgenic
ES Cell Line: Not applicable
Mice that are homozygous for the targeted mutation and hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These double mutant mice produce mutated human tau (MAPT), but do not produce mouse tau (Mapt). The ht-PAC-305S transgenic mouse expresses all 6 human tau isoforms with a 3 repeat (3R)-tau to 4 repeat (4R) -tau ratio of approximately 80% 4R-tau to 20% 3R-tau rather than the expected human ratio of 1:1. S305S is associated with frontotemporal dementia with parkinsonism linked to chromosome 17 (FTDP 17) and is believed to increase exon 10 inclusion during splicing by strengthening the weak exon 10 5' splice site. No gross or microscopic pathologic lesions were observed in these mice. This mutant mouse strain may be useful in studies of tau isoforms in neurodegenerative disease.
Heterozygous mice have not been evaluated.
Strain of Origin: C57BL6/C3H/129X1
Strain genetic background: B6.Cg
Strain Development: This transgene was microinjected into fertilized C57BL/6 x C3H hybrid oocytes and founder mice were backcrossed to C57BL/6 mice for approximately 15 generations. This transgenic line was crossed to B6.129-Mapttm1Hnd mice to generate mice that were homozygous for the targeted mutation and hemizygous for the transgene. The colony has been maintained by sibling mating.
Suggested Control Mice: Wild-type littermates homozygous for the knockout and wild-type for the transgene.
Donor: Gerard Schellenberg, Ph.D., University of Pennsylvania
McMillan P; Korvatska E; Poorkaj P; Evstafjeva Z; Robinson L; Greenup L; Leverenz J; Schellenberg GD; D'Souza I, Tau isoform regulation is region- and cell-specific in mouse brain. J Comp Neurol 2008 Dec 20;511(6):788-803 (Medline PMID: 18925637)
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email firstname.lastname@example.org.
Coat color: Black
MMRRC Breeding System: Sib-mating. While maintaining a live colony, these mice are bred as homozygous for the targeted mutation and hemizygous for the transgene.
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes||Mice that are homozygous for both the KO and Tg are viable.|
|Homozygotes are fertile:||Yes||Yes||Mice that are homozygous for the KO and hemizygous for the Tg are fertile|
|Hetero/Hemizygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 5
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.